Tribo™ Human IFN γ ELISA Kit

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Tribo™ Human IFN γ ELISA Kit

$390.00

The Tribo™ Fast Human IFN-γ ELISA Maxi is a solid phase ELISA designed to measure human IFN- γ levels in cell culture supernatants, serum, and plasma.

SKU: TBS3230 Category: Tag:
SKUStock SIZE PriceQuantity
TBS3230Yes1x96-well plate $390.00
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Description

The Tribio® Fast Human IFN- γ ELISA Maxi is a solid phase ELISA designed to measure human IFN- γ levels in cell culture supernatants, serum, and plasma. The main feature is that the kit uses our novel proprietary approaches to combine samples and detection into a one-step instead of the complicated traditional methods. It makes the assay simple, easy, accurate, and fast. The measurement can be finished in 1 hour, not need 5-6 hours (Fig. 1). The measurement can be finished within 1.0 hour, not need 4-5 hours. The detection arrange is from 20 to 15000pg/mL. The levels of human IFN- γ samples are parallel to the standard curves obtained using the kit standards linearly. These results indicate that this kit can be used to determine relative mass values for natural human IFN- γ protein.

Sensitivity

The minimum detectable dose (MOD) of human IFN-γ is typically 8 pg/ml.

 

Specificity

This assay recognizes natural and recombinant human IFN- γ.

 

Simple Procedures

Simple Standard Preparation

Accurate Standard Curve

Kit Contents & Storage Conditions:

PartPart #DescriptionStorage of Opened/Reconstituted
Human IFN-γ MicroplateTBS3230A96 well strip microplate (12 strips of 8 wells) coated with a polyclonal antibody specific for human IFN- γ.Return unused wells to the foil pouch containing the desiccant pack. Reseal along the entire edge of the zip-seal. May be stored for up to 1 month at 2-8 °C.
Human IFN-γ StandardTBS3230B100 µL of Recombinant human IFN- γ protein (145.8ng/mL).Aliquot and store at -20 ℃ for up to 1 month in a manual defrost the freezer. Avoid repeated freeze-thaw cycles.
Detection ATBS3230C2.2 ml of HRP-Human IFN- γ antibody.May be stored for up to 3 months at 2-8℃
Assay DiluentTBS3230D12 ml of a buffered protein base with preservatives.
Wash BufferTBS3000W12 ml of concentrated solution (10x).
TMB SubstrateTBS3000T12 ml of ultra-sensitive TMB substrate.
Stop SolutionTBS3000S6 ml of 2 N sulfuric acid.

Store the unopened kit at 2-8 ℃. Do not use past kit expiration date.
The kit contains sufficient materials to run an ELISA on one 96 well plate.

 

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Additional information

SIZE

1×96-well plate

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