Tribo™ Human Complement C4 ELISA

Description

The Fast Human Complement C4 ELISA is a solid phase ELISA designed to measure human complement C4 levels in cell culture supernatants, serum, and plasma. The main feature is that the kit uses our novel proprietary approaches to combine samples and detections into a one-step instead of the complicated traditional methods. It makes the assay simple, easy, accurate, and fast. The measurement can be finished in 1 hour, not need 4-5 hours (Fig. 1). The detection range is from 1 to 300 ng/mL. The levels of human complement C4 samples are parallel to the standard curves obtained using the kit standards linearly. These results indicate that this kit can be used to determine relative mass values for natural human complement C4 protein.

 

Kit Contents and Storage Conditions:

Reagents	Quantity
Human Complement C4 Microplate	96 well microplate (12 strips of 8 wells) coated with a Capture Antibody specific for human complement C4.
Human Complement C4 Standard	100 µL of Recombinant human complement C4 protein (3 µg/mL).
Detection A	2.1 ml of human Complement C4 antibody-Biotin.
Detection B	100 µL ml of Streptavidin-HRP (100x).
Assay Diluent	20 ml of a buffered protein base with preservatives.
10x WashBuffer	12ml of concentrated solution(10x).
TMB Substrate	12 ml of ultra-sensitive TMB substrate.
Stop Solution	6ml of 2 N sulfuric acid.

Store the unopened kit at 2-8 ℃. Do not use past kit expiration date.
The kit contains sufficient materials to run an ELISA on one 96 well plate.

 

Fig. 1 Simple Assays Procedures

Fig.2 Diagram for Human Complement C4 Standard Preparation

Fig.3 Standard Curve

 

Related Products:

1. Human IL-1 ß; ELISA; 1 step and 1hr (TBS3219)
2. Human IL-2 ELISA; 1 step and 1hr (TBS3220)
3. Human IL-4 ELISA; 1 step and 1hr (TBS3221)
4. Human IL-6 ELISA; 1 step and 1hr (TBS3223)
5. Human IL-7 ELISA; 1 step and 1hr (TBS3224)

 

Additional information

SIZE (kit)	1 x 96-Well Plate

Datasheet:

TBS3201_human_complement c4_elisa_V02_04_2021

MSDS:

TBS3201_Human_Complement_C4_ELISA_MSDS