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Tribo™ Human Complement C4 ELISA
Complement component 4 (C4) plays a key role in the activation of the classical complement pathway. C4 is synthesized as a single-chain precursor molecule (200 kDa) but processed to the three-chain structure with alpha (93 kDa), beta (78 kDa), and gamma (33 kDa) chains prior to secretion. After activation by C1s, C4 is processed to C4a and C4b. C4a anaphylatoxin is a mediator of local inflammation and induces smooth muscle contraction. C4b, the major activation product, is an essential subunit of the C3 and C5 convertases of the classical complement pathway. C4 deficiency is associated with systemic lupus erythematosus. The C4b degradation product, C4d, is a marker for humoral rejection in allografts.
The Fast Human Complement C4 ELISA is a solid phase ELISA designed to measure human complement C4 levels in cell culture supernatants, serum, and plasma. The main feature is that the kit uses our novel proprietary approaches to combine samples and detections into a one-step instead of the complicated traditional methods. It makes the assay simple, easy, accurate, and fast. The measurement can be finished in 1 hour, not need 4-5 hours (Fig. 1). The detection arrangement is from 1 to 300 ng/mL. The levels of human complement C4 samples are parallel to the standard curves obtained using the kit standards linearly. These results indicate that this kit can be used to determine relative mass values for natural human complement C4 protein.
Kit Contents and Storage Conditions:
|Human Complement C4 Microplate||96 well microplate (12 strips of 8 wells) coated with a|
Capture Antibody specific for human complement C4.
|Human Complement C4 Standard||100 µL of Recombinant human complement C4 protein|
|Detection A||2.1 ml of human Complement C4 antibody-Biotin.|
|Detection B||100 µL ml of Streptavidin-HRP (100x).|
|Assay Diluent||20 ml of a buffered protein base with preservatives.|
|10x WashBuffer||12ml of concentrated solution(10x).|
|TMB Substrate||12 ml of ultra-sensitive TMB substrate.|
|Stop Solution||6ml of 2 N sulfuric acid.|
Store the unopened kit at 2-8 ℃. Do not use past kit expiration date.
The kit contains sufficient materials to run an ELISA on one 96 well plate.
Fig. 1 Simple Assays Procedures
Fig.2 Diagram for Human Complement C4 Standard Preparation
Fig.3 Standard Curve
- Human IL-1 ß; ELISA; 1 step and 1hr (TBS3219)
- Human IL-2 ELISA; 1 step and 1hr (TBS3220)
- Human IL-4 ELISA; 1 step and 1hr (TBS3221)
- Human IL-6 ELISA; 1 step and 1hr (TBS3223)
- Human IL-7 ELISA; 1 step and 1hr (TBS3224)
1 X 96-Well Plate