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Fast Mouse GM-CSF ELISA
Granulocyte-Macrophage Colony Stimulating Factor (GM-CSF), also known as CSF-2, is a pleiotropic 30 kDa member of the Common beta Chain (βc) cytokine family that also includes IL-3 and IL-5. It is secreted by a wide variety of activated immune, mesenchymal, and epithelial cell types and circulates as a variably glycosylated monomer. It is upregulated in multiple cell types during inflammation including encephalitogenic T cells, allergen exposed lung endothelial cells, and IgE activated mast cells. It induces monocyte, neutrophil, and eosinophil production from CD34+ stem cell precursors. GM-CSF promotes Th1 and Th17 cell-mediated autoimmune inflammation as well as the inflammatory activation of dendritic cells, microglia, alveolar macrophages, and eosinophils. In addition, it cooperates with G-CSF in promoting tumor cell proliferation and invasion.
The Mouse GM-CSF ELISA is designed to quantitatively detect Mouse GM-CSF levels in different tissues including skin, muscle, neural, serum, and other biological samples. The main feature is that the kit uses our novel proprietary approaches to combine samples and detections into a one-step instead of the complicated traditional methods. It makes the assay simple, easy, accurate, and fast. The measurement can be finished in 2 hours, not need 4-5 hours (Fig. 1). The detection arrangement is from 7.8 to 500pg/mL. The levels of GM-CSF samples are parallel to the standard curves obtained using the kit standards linearly. These results indicate that this kit can be used to determine relative mass values for natural GM-CSF protein.
Kit Contents and Storage Conditions:
|Mouse GM-CSF Capture||96 well microplate (12 strips of 8 wells) coated with a polyclonal antibody specific for mouse GM-CSF.|
|Mouse GM-CSF Standard||10 µl of Recombinant mouse GM-CSF protein (80ng/mL).|
|Detection A||2.1 mL of Biotin-mouse GM-CSF antibody.|
|Detection B||200 µl of Streptavidin-HRP (50x).|
|Assay Diluent||20 ml of a buffered protein base with preservatives.|
|WashBuffer||12 ml of concentrated solution (10x).|
|TMB Substrate||12 ml of ultra-sensitive TMB substrate.|
|Stop Solution||6 ml of 2 N sulfuric acid.|
Store the unopened kit at 2-8 ℃. Do not use past kit expiration date.
The kit contains sufficient materials to run an ELISA on a 3x 96 well plate.
Fig. 1 Simple Assays Procedures
Fig. 2 Diagram for Mouse GM-CSF Standard Preparation
Fig3. Mouse GM-CSF Standard
1 X 96-Well Plate