Ultra-sensitive Human IL-33 ELISA
This is ultra-sensitive ELISA and can detect the concentration as low as 0.5pg/mL in cell culture Supernate, Plasma/Serum IL-33.
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Interleukin-33 (IL-33), also known as NF-HEV and DVS 27, is a 30 kDa pro-inflammatory protein. Human IL-33 is synthesized as a 270 amino acid molecule with an N-terminal nuclear localization signal, a helix-turn-helix motif, and a C-terminal region with structural homology to IL-1 family cytokines. IL-33 exerts multiple effects on immune system function. It acts on Th2 cells, basophils, and mast cells to induce their migration to sites of inflammation and the production of Th2 cytokines. IL-33 also promotes the expansion of regulatory T cells and alternately activated macrophages while attenuating Th17 cell expansion and activation. IL-33 contributes to infection clearance by enhancing neutrophil sensitization to TLR and Dectin-1 signaling, phagocytic activity, and migration to sites of infection. It is upregulated in a wide variety of cells under inflammatory conditions.
The Tribio® Ultra-sensitive Human IL-33 Fast ELISA is a solid phase ELISA designed to measure human IL-33 levels in cell culture supernatants, serum, and plasma. The main feature is that the kit uses our novel proprietary approaches to combine samples and detection into a one-step instead of the complicated traditional methods. It makes the assay simple, easy, accurate, and ultra-sensitive. The measurement can detect the concentration as low as 0.5pg/mL, and finished in 2 hours, not need 5-6 hours (Fig. 1). The detection arrangement is from 0.5 to 2048 pg/mL. The levels of human IL-33 samples are parallel to the standard curves obtained using the kit standards linearly. Therefore, the kit can be used to determine relative mass values for natural human IL-33 protein.
Kit Content and Storage Conditions
|Part||Part#||Description||Storage of Opened/Reconstituted|
|Human IL-33 Microplate||TBS3245A||96 well strip microplate (12 strips of 8 wells) coated with a polyclonal antibody specific for human IL-33.||Return unused wells to the foil pouch containing the desiccant pack. Reseal along the entire edge of the zip-seal. May be stored for up to 1 month at 2-8 °C.|
|Human IL-33 Standard||TBS3245B||100 µL of Recombinant human IL-33 protein (100 ng/mL).||Aliquot and store at -20 ℃ for up to 1 month in a manual defrost the freezer. Avoid repeated freeze-thaw cycles.|
|Detection A||TBS3245C||2.2 ml of HRP-Human IL-33 antibody.||May be stored for up to 3 months at 2-8 °C.|
|Assay Diluent||TBS3245D||12 ml of a buffered protein base with preservatives.|
|Wash Buffer||TBS3000W||12 mL of concentrated solution (10x).|
|TMB Substrate||TBS3000T||12 mL of ultra-sensitive TMB substrate.|
|Stop Solution||TBS3000S||6 mL of 2 N sulfuric acid.|
Store the unopened kit at 2-8 ℃. Do not use past kit expiration dates.
The kit contains sufficient materials to run an ELISA on one 96 well plate.
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