Human Total beta-Catenin ELISA (TBS32130)
$490.00
Synonyms: armadillo; Beta-catenin; catenin (cadherin-associated protein), beta 1, 88kDa; catenin beta 1; Catenin beta-1; CTNB1; CTNNB; CTNNB1; DKFZp686D02253; EVR7; FLJ25606; FLJ37923; MRD19; NEDSDV
| SKU | Stock | SIZE | Price | Quantity | ||
|---|---|---|---|---|---|---|
| TBS32130 | Yes | 96-well plate | $490.00 |
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Description
Tribioscience’s Human Beta-Catenin ELISA is designed to quantitatively detect human beta-Catenin levels in serum, plasma, and other biological samples. The main feature is that the kit uses our novel proprietary approaches to combine samples and detections into a one-step instead of the complicated traditional methods. It makes the assay simple, easy, accurate and fast. The Hands-on time can be within 2 hours, not need 4-5 hours (Fig. 1). The detection range is from 312 to 20000 pg/mL. The levels of human beta-Catenin samples are parallel to the standard curves obtained using the kit standards linearly. Therefore, the kit can be used to determine relative mass values for natural human beta-Catenin protein.
Typical Data
This standard curve (R2=1.000) is provided for demonstration only. A standard curve should be generated for each set of samples assayed. Fig. 3 is an example of typical Data.
Sensitivity
The minimum detectable dose (MDD) of human beta-Catenin is typically 10 pg/mL.
The Intra-assay CV and the Inter-assay CV are ˂10%.
Specificity
This assay recognizes natural and recombinant human beta-Catenin.
No cross-reactivity with others.
Simple Procedures (Fig. 1)
Simple Standard Preparation
Standard Curve
Kit Content and Storage Conditions
| PART | PART# | DESCRIPTION | STORAGE OF OPENED/ RECONSTITUTED |
| Human beta-Catenin Microplate | TBS32130A | 96 well polystyrene microplate (12 strips of 8 wells) coated with a monoclonal antibody specific for human beta-Catenin. | Return unused wells to the foil pouch. Reseal along the entire edge of the zip-seal. May be stored for up to 1 month at 2-8 °C. |
| Human beta-Catenin Standard | TBS32130B | 50 µl of Recombinant human beta-Catenin (400 ng/mL). | Aliquot and store at -20 ℃ for up to 1 month in a manual defrost the freezer. Avoid repeated freeze-thaw cycles. |
| Detection A | TBS32130C | 2.1 mL of biotin- human beta-Catenin antibody. |
May be stored for up to 3 months at 2-8 °C. |
| Detection B | TBS32130D | 400 µL of streptavidin HRP. | |
| Assay Diluent | TBS32130E | 25 mL of a buffered protein base with preservatives. | |
| Wash Buffer | TBS3000W | 12 mL of concentrated solution (10x) | |
| TMB Substrate | TBS3000T | 12 mL of ultra-sensitive TMB substrate. | |
| Stop Solution
|
TBS3000S | 6 mL of 2 N sulfuric acid. |
Store the unopened kit at 2-8 ℃. Do not use past kit expiration date.
The kit contains sufficient materials to run an ELISA on one 96 well plate.
Research use only.
Additional information
| SIZE | 96-well plate |
|---|
Datasheet:
TBS32130_Human Total beta-Catenin-ELISA_datasheet
MSDS:
TBS32130_Human Total Beta-Catenin ELISA_MSDS
Research use only.
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