Tryptase ELISA (TBS32131)
$560.00
Synonyms: TPSAB1; TPS;
| SKU | Stock | SIZE | Price | Quantity | ||
|---|---|---|---|---|---|---|
| TBS32131 | Yes | 96-well plate | $560.00 |
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Description
Tribioscience’s Tryptase ELISA is designed to quantitatively detect tryptase levels in serum, plasma, and other biological samples. The main feature is that the kit uses our novel proprietary approaches to combine samples and detections into a one-step instead of the complicated traditional methods. It makes the assay simple, easy, accurate and fast. The hands-on time can be within 2 hours, not need 4-5 hours (Fig. 1). The detection range is from 0.16 to 10 ng/mL. The levels of tryptase samples are parallel to the standard curves obtained using the kit standards linearly. Therefore, the kit can be used to determine relative mass values for natural tryptase protein.
Typical Data
This standard curve (R2=1.000) is provided for demonstration only. A standard curve should be generated for each set of samples assayed. Fig. 3 is an example of typical Data.
Sensitivity
The minimum detectable dose (MDD) of tryptase is typically 1 ng/mL.
The Intra-assay CV and the Inter-assay CV are ˂10%.
Specificity
This assay recognizes natural and recombinant tryptase.
No cross-reactivity with others.
Simple Procedures (Fig. 1)
Simple Standard Preparation
Standard Curve
Kit Content and Storage Conditions
| PART | PART# | DESCRIPTION | STORAGE OF OPENED/ RECONSTITUTED |
| Tryptase Microplate | TBS32131A | 96 well polystyrene microplate (12 strips of 8 wells) coated with a monoclonal antibody specific for tryptase. | Return unused wells to the foil pouch. Reseal along the entire edge of the zip-seal. May be stored for up to 1 month at 2-8 °C. |
| Tryptase Standard | TBS32131B | 50 µl of Recombinant tryptase (200 ng/mL). | Aliquot and store at -20 ℃ for up to 1 month in a manual defrost the freezer. Avoid repeated freeze-thaw cycles. |
| Detection A | TBS32131C | 2.1 mL of biotin-tryptase antibody. |
May be stored for up to 3 months at 2-8 °C. |
| Detection B | TBS32131D | 400 µL of streptavidin HRP. | |
| Assay Diluent | TBS32131E | 25 mL of a buffered protein base with preservatives. | |
| Wash Buffer | TBS3000W | 12 mL of concentrated solution (10x) | |
| TMB Substrate | TBS3000T | 12 mL of ultra-sensitive TMB substrate. | |
| Stop Solution | TBS3000S | 6 mL of 2 N sulfuric acid. |
Store the unopened kit at 2-8 ℃. Do not use past kit expiration date.
The kit contains sufficient materials to run an ELISA on one 96 well plate.
Research use only.
Additional information
| SIZE | 96-well plate |
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