Botulinum Neurotoxin Type E (BoNT-E) ELISA (TBS31009)
$580.00
Botulinum Neurotoxin E
| SKU | Stock | SIZE | Price | Quantity | ||
|---|---|---|---|---|---|---|
| TBS31009 | Yes | 1 x 96-well plate | $580.00 |
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Description
Tribioscience’s Botulinum Neurotoxin Type E (BoNT-E) ELISA is designed to quantitatively detect Botulinum Neurotoxin Type E (BoNT-E) levels in serum, plasma, and other biological samples. The main feature is that the kit uses our novel proprietary approaches to combine samples and detections into a one-step instead of the complicated traditional methods. It makes the assay simple, easy, accurate, and fast (Fig. 1). The detection range is from 62.5 to 4000 pg/mL. The levels of Botulinum Neurotoxin Type E (BoNT-E) samples are parallel to the standard curves obtained using the kit standards linearly. Therefore, the kit can be used to determine relative mass values for natural Botulinum Neurotoxin Type E (BoNT-E) protein.
Typical Data
This standard curve (R2=0.9972) is provided for demonstration only. A standard curve should be generated for each set of samples assayed. Fig. 3 is an example of typical Data.
Sensitivity
The minimum detectable dose (MDD) of BoNT-E is typically 46 pg/mL.
The Intra-assay CV and the Inter-assay CV are ˂10%.
Specificity
This assay recognizes natural and recombinant BoNT-E.
No cross-reactivity with others.
Simple Procedures (Fig. 1)
Simple Standard Preparation
Accurate Standard Curve
Kit Content and Storage Conditions
| PART | PART# | DESCRIPTION | STORAGE OF OPENED/ RECONSTITUTED |
| Botulinum Neurotoxin Type E Microplate | TBS31009A | 96 well polystyrene microplate (12 strips of 8 wells) coated with a monoclonal antibody specific for BoNT-E. | Return unused wells to the foil pouch. Reseal along the entire edge of the zip-seal. May be stored for up to 1 month at 2-8 °C. |
| Botulinum Neurotoxin Type E Standard | TBS31009B | 50 µl of Recombinant Botulinum Neurotoxin Type E (40 ng/mL). | Aliquot and store at -20 ℃ for up to 1 month in a manual defrost freezer. Avoid repeated freeze-thaw cycles. |
| Detection A | TBS31009C | 2.1 mL of biotin-Botulinum Neurotoxin Type E antibody. |
May be stored for up to 3 months at 2-8 °C. |
| Detection B | TBS31009D | 200 µL of streptavidin HRP. | |
| Assay Diluent | TBS31009E | 25 mL of a buffered protein base with preservatives. | |
| Wash Buffer | TBS3000W | 12 mL of concentrated solution (10x). | |
| TMB Substrate | TBS3000T | 12 mL of ultra-sensitive TMB substrate. | |
| Stop Solution | TBS3000S | 6 mL of 2 N sulfuric acid. |
Store the unopened kit at 2-8 ℃. Do not use past kit expiration dates.
The kit contains sufficient materials to run an ELISA on one 96-well plate.
Research use only.
Additional information
| SIZE | 1 x 96-well plate |
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