Human TNFꭤ ELISA kit; 1 step and 1hr

$390.00

The TriboTM Fast Human TNF-α ELISA is designed to quantitatively detect Human TNF-α levels in different tissues including skin, muscle, neural, serum, and other biological samples.

SKU: TBS3235 Category:
SKUStock SIZE PriceQuantity
TBS3235 Yes 1 x 96-well plate $390.00
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Description

The TriboTM Fast Human TNF-α ELISA is designed to quantitatively detect Human TNF-α levels in different tissues including skin, muscle, neural, serum, and other biological samples. The main feature is that the kit uses our novel proprietary approaches to combine samples and detections into a one-step instead of the complicated traditional methods. It makes the assay simple, easy, accurate, and fast. The measurement can be finished in 3 hours, with no need for 4-5 hours (Fig. 1). The detection range is from 8 to 2000 pg/mL. The levels of human TNF-α samples are parallel to the standard curves obtained using the kit standards linearly. These results indicate that this kit can be used to determine relative mass values for natural human TNF-α protein.

Key Features

  • Novel Proprietary ELISA Approach
  • Fast: Only 1 hour,  not 4-5 hrs.
  • Simple: Sample and Detection combined into one step.
  • Easy: All procedures are performed at room temperature.
  • Broad Detection Range: The detection range is from 15 to 1000 pg/mL.

The levels of human TNF-α samples are parallel to the standard curves obtained using the kit standards linearly. These results indicate that this kit can be used to determine relative mass values for natural human TNF-α protein.

Typical Data

This standard curve(R2=0.9995) is provided for demonstration only. A standard curve should be generated for each set of samples assayed. Fig. 3 is an example of typical Data.

Sensitivity

The minimum detectable dose (MOD) of human TNF-α is typically 10 pg/ml. The Intra-assay CV is 3.79% the Inter-assay CV is ˂10%.

Specificity

This assay recognizes natural and recombinant human TNF-α.

No cross-reactivity: human TNF-β; human TNF RI, human TNF RII; Porcine TNF-α; Rat TNF-α; Mouse TNF-α; Mouse TNF RI, Mouse TNF RII.

 

Simple Procedures (Fig. 1)

 

Simple Standard Preparation

 

Accurate Standard Curve

Kit Content and Storage Conditions

Part Part# Description Storage of Opened/Reconstituted
Human TNF-α Microplate TBS3235A 96-well polystyrene microplate (12 strips of 8 wells) coated with a polyclonal antibody specific for human TNF-α. Return unused wells to the foil pouch. Reseal along the entire edge of the zip-seal. May be stored for up to 1 month at 2-8 °C.
Human TNF-α Standard TBS3235B 30 µl of Recombinant human TNF-α protein (100 ng/mL). Aliquot and store at -20 ℃ for up to 1 month in a manual defrost the freezer. Avoid repeated freeze-thaw cycles.
Detection A TBS3235C 2.1 ml of Biotin-Human TNF-α antibody. May be stored for up to 3 months at 2-8 °C.
Detection B TBS3235D 12 ml of Streptavidin-HRP.
Assay Diluent TBS3235E 12 ml of a buffered protein base with preservatives.
Wash Buffer TBS3000W 12 mL of concentrated solution (10x).
TMB Substrate TBS3000T 12 mL of ultra-sensitive TMB substrate.
Stop Solution TBS3000S 6 mL of 2 N sulfuric acid.

Store the unopened kit at 2-8 ℃. Do not use past kit expiration dates.
The kit contains sufficient materials to run an ELISA on one 96-well plate.

Related Product

  1. Human IL-1ß ELISA (TBS3219)
  2. Human IL-2 ELISA (TBS3220)
  3. Human IL-4 ELISA (TBS3221)
  4. Human IL-6 ELISA (TBS3223)
  5. Human IL-7 ELISA (TBS3224)

 

Additional information

SIZE

1 x 96-well plate

Ao, Z., Wu, Z., Cai, H., Hu, L., Li, X., Kaurich, C., Chang, J., Gu, M., Cheng, L., Lu, X., & Guo, F. (2022). Rapid Profiling of Tumor-Immune Interaction Using Acoustically Assembled Patient-Derived Cell Clusters. Advanced science (Weinheim, Baden-Wurttemberg, Germany), 9(22), e2201478. https://doi.org/10.1002/advs.202201478

1 review for Human TNFꭤ ELISA kit; 1 step and 1hr

  1. 5 out of 5

    Zheng Ao

    Used it for microglia assay, very fast and sensitive. Easy to handle, and all reagents are included. Would recommend.


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