Human TNFꭤ ELISA kit; 1 step and 1hr

Human TNFꭤ ELISA kit; 1 step and 1hr

$390.00

The TriboTM Fast Human TNF-α ELISA is designed to quantitatively detect Human TNF-α levels in different tissues including skin, muscle, neural, serum, and other biological samples.

SKU: TBS3235 Category:
SKUStock SIZE PriceQuantity
TBS3235Yes1x96-well plate $390.00
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Description

Tumor necrosis factor alpha (TNF-α,also known as TNFSF2) is a potent mediator of immune and inflammatory response. It is produced by many activated cell types including monocytes, macrophages, astrocytes, granulocytes, T and B lymphocytes, NK cells, keratinocytes, fibroblasts, and certain tumor cells. TNF-α is involved in a number of pathological conditions including inflammation, apoptosis, lipid metabolism, trauma, asthma, rheumatoid arthritis, pain, obesity septic shock, autoimmunity, and cancer.

The Tribo Fast Human TNF-α ELISA is designed to quantitatively detect Human TNF-α levels in different tissues including skin, muscle, neural, serum, and other biological samples. The main feature is that the kit uses our novel proprietary approaches to combine samples and detections into a one-step instead of the complicated traditional methods. It makes the assay simple, easy, accurate and fast. The measurement can be finished in 1 hour, not need 4-5 hours (Fig.1). The detection arrange is from 15 to 1000pg/mL. The levels of human TNF-α samples are parallel to the standard curves obtained using the kit standards linearly. These results indicate that this kit can be used to determine relative mass values for natural human TNF-α protein.

Key Features:

  • Novel Proprietary ELISA Approach
  • Fast: Only 1 hour,  not 4-5 hrs.
  • Simple: Sample and Detection combined into one-step.
  • Easy: All procedures are performed at room temperature.
  • Broad Detection Range: The detection range is from 15 to 1000 pg/mL.

The levels of human TNF-α samples are parallel to the standard curves obtained using the kit standards linearly. These results indicate that this kit can be used to determine relative mass values for natural human TNF-α protein.

Principle of the Assay

Kit Content and Storage Condition:


Store the unopened kit at 2-8℃. Do not use past kit expiration date.

Typical Data

This standard curve(R2=0.9995) is provided for demonstration only. A standard curve should be generated for each set of samples assayed. Fig. 3 is an example of typical Data.

Sensitivity

The minimum detectable dose (MOD) of human TNF-α is typically 10pg/ml. The Intra-assay CV is 3.79% the Inter-assay CV is ˂10%.

Specificity

This assay recognizes natural and recombinant human TNF-α.

No cross-reactivity: human TNF-β; human TNF RI, human TNF RII; Porcine TNF-α; Rat TNF-α; Mouse TNF-α; Mouse TNF RI, Mouse TNF RII.

Additional information

SIZE

1×96-well plate

1 review for Human TNFꭤ ELISA kit; 1 step and 1hr

  1. 5 out of 5

    Zheng Ao

    Used it for microglia assay, very fast and sensitive. Easy to handle, and all reagents are included. Would recommend.


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