Human Insulin ELISA; 1 step and 1hr

$390.00

SKU: TBS3236 Categories: ,
SKUStock SIZE PriceQuantity
TBS3236 Yes 1 x 96-well plate $390.00
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Description

Tribioscience’s Fast Human Insulin ELISA is designed to quantitatively detect human insulin levels in different tissues including skin, muscle, neural, serum, and other biological samples. The main feature is that the kit uses our novel proprietary approaches to combine samples and detections into a one-step instead of the complicated traditional methods. It makes the assay simple, easy, accurate, and fast. The measurement can be finished in 1 hour, with no need for 4-5 hours (Fig. 1). The detection range is from 51.2 to 12500 pg/mL. The levels of human insulin samples are parallel to the standard curves obtained using the kit standards linearly. These results indicate that this kit can be used to determine relative mass values for natural human insulin.

 

Typical Data

This standard curve(R2=0.9998) is provided for demonstration only. A standard curve should be generated for each set of samples assayed. Fig. 3 is an example of typical Data.

Sensitivity

The minimum detectable dose (MOD) of human is typically 20pg/ml. The Intra-assay CV is 4.79% the Inter-assay CV is ˂10%.

Specificity

This assay recognizes natural and recombinant human insulin.

 

Simple Procedures

 

Simple Standard Preparation

 

Accurate Standard Curve

 

 

Kit Content and Storage Conditions

PART PART# DESCRIPTION STORAGE OF OPENED/ RECONSTITUTED
Human Insulin Microplate TBS3236A 96-well polystyrene microplate (12 strips of 8 wells) coated with a monoclonal antibody specific for human insulin. Return unused wells to the foil pouch. Reseal along the entire edge of the zip-seal. May be stored for up to 1 month at 2-8 °C.
Human Insulin Standard TBS3236B 100 µl of Recombinant human insulin (100ng/mL). Aliquot and store at -20 ℃ for up to 1 month in a manual defrost freezer. Avoid repeated freeze-thaw cycles.
Detection A TBS3236C 2.2 ml of Biotin- human insulin antibody.  

May be stored for up to

3 months at 2-8 °C.*

Detection B TBS3236D 12 ml of Streptavidin-HRP.
Assay Diluent TBS3000E 12 ml of a buffered protein base with preservatives.
Wash Buffer TBS3000W 12 ml of concentrated solution (10x).
TMB Substrate TBS3000T 12 ml of ultra-sensitive TMB substrate.
Stop Solution TBS3000S 6 ml of 2 N sulfuric acid.

Store the unopened kit at 2-8 ℃. Do not use past kit expiration dates.

The kit contains sufficient materials to run an ELISA on one 96-well plate.

 

Related Product

  1. Human IL-1ß ELISA (TBS3219)
  2. Human IL-2 ELISA (TBS3220)
  3. Human IL-4 ELISA (TBS3221)
  4. Human IL-6 ELISA (TBS3223)
  5. Human IL-7 ELISA (TBS3224)

 

Additional information

SIZE

1 x 96-well plate

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