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Insulin is a peptide hormone of the insulin-like peptide family. It is produced by pancreatic β cells and is essential for glucose metabolism and regulation of energy balance. Failure of insulin control causes diabetes mellitus (DM), which can either be of Type I (T1D, 5% of diagnosed DM) or Type II (T2D, 95% of diagnosed DM). T1D is a primary insufficiency of β cell insulin production while T2D is a functional insulin deficiency caused mainly by insulin resistance of the target cells. DM has become more frequent over time and is the seventh leading cause of death in the US.
Tribioscience’s Fast Human Insulin ELISA is designed to quantitatively detect human insulin levels in different tissues including skin, muscle, neural, serum, and other biological samples. The main feature is that the kit uses our novel proprietary approaches to combine samples and detections into a one-step instead of the complicated traditional methods. It makes the assay simple, easy, accurate, and fast. The measurement can be finished in 1 hour, not need 4-5 hours (Fig. 1). The detection arrange is from 51.2 to 12500pg/mL. The levels of human insulin samples are parallel to the standard curves obtained using the kit standards linearly. These results indicate that this kit can be used to determine relative mass values for natural human insulin.
KIT CONTENT AND STORAGE CONDITIONS
|PART||PART#||DESCRIPTION||STORAGE OF OPENED/ RECONSTITUTED|
|Human Insulin Microplate||TBS3236A||96 well polystyrene microplate (12 strips of 8 wells) coated with a monoclonal antibody specific for human insulin.||Return unused wells to the foil pouch. Reseal along the entire edge of the zip-seal. May be stored for up to 1 month at 2-8 °C.|
|Human Insulin Standard||TBS3236B||100 µl of Recombinant human insulin (100ng/mL).||Aliquot and store at -20 ℃ for up to 1 month in a manual defrost the freezer. Avoid repeated freeze-thaw cycles.|
|Detection A||TBS3236C||2.2 ml of Biotin- human insulin antibody.|| |
May be stored for up to
3 months at 2-8 °C.*
|Detection B||TBS3236D||12 ml of Streptavidin-HRP.|
|Assay Diluent||TBS3000E||12 ml of a buffered protein base with preservatives.|
|Wash Buffer||TBS3000W||12 ml of concentrated solution (10x).|
|TMB Substrate||TBS3000T||12 ml of ultra-sensitive TMB substrate.|
|Stop Solution||TBS3000S||6 ml of 2 N sulfuric acid.|
Store the unopened kit at 2-8 ℃. Do not use past kit expiration dates.
The kit contains sufficient materials to run an ELISA on one 96 well plates.
- Human IL-1ß ELISA (TBS3219)
- Human IL-2 ELISA (TBS3220)
- Human IL-4 ELISA (TBS3221)
- Human IL-6 ELISA (TBS3223)
- Human IL-7 ELISA (TBS3224)