Human Insulin ELISA; 1 step and 1hr
$390.00
SKU | Stock | SIZE | Price | Quantity | ||
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TBS3236 | Yes | 1 x 96-well plate | $390.00 |
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Description
Tribioscience’s Fast Human Insulin ELISA is designed to quantitatively detect human insulin levels in different tissues including skin, muscle, neural, serum, and other biological samples. The main feature is that the kit uses our novel proprietary approaches to combine samples and detections into a one-step instead of the complicated traditional methods. It makes the assay simple, easy, accurate, and fast. The measurement can be finished in 1 hour, with no need for 4-5 hours (Fig. 1). The detection range is from 51.2 to 12500 pg/mL. The levels of human insulin samples are parallel to the standard curves obtained using the kit standards linearly. These results indicate that this kit can be used to determine relative mass values for natural human insulin.
Typical Data
This standard curve(R2=0.9998) is provided for demonstration only. A standard curve should be generated for each set of samples assayed. Fig. 3 is an example of typical Data.
Sensitivity
The minimum detectable dose (MOD) of human is typically 20pg/ml. The Intra-assay CV is 4.79% the Inter-assay CV is ˂10%.
Specificity
This assay recognizes natural and recombinant human insulin.
Simple Procedures
Simple Standard Preparation
Accurate Standard Curve
Kit Content and Storage Conditions
PART | PART# | DESCRIPTION | STORAGE OF OPENED/ RECONSTITUTED |
Human Insulin Microplate | TBS3236A | 96-well polystyrene microplate (12 strips of 8 wells) coated with a monoclonal antibody specific for human insulin. | Return unused wells to the foil pouch. Reseal along the entire edge of the zip-seal. May be stored for up to 1 month at 2-8 °C. |
Human Insulin Standard | TBS3236B | 100 µl of Recombinant human insulin (100ng/mL). | Aliquot and store at -20 ℃ for up to 1 month in a manual defrost freezer. Avoid repeated freeze-thaw cycles. |
Detection A | TBS3236C | 2.2 ml of Biotin- human insulin antibody. |
May be stored for up to 3 months at 2-8 °C.* |
Detection B | TBS3236D | 12 ml of Streptavidin-HRP. | |
Assay Diluent | TBS3000E | 12 ml of a buffered protein base with preservatives. | |
Wash Buffer | TBS3000W | 12 ml of concentrated solution (10x). | |
TMB Substrate | TBS3000T | 12 ml of ultra-sensitive TMB substrate. | |
Stop Solution | TBS3000S | 6 ml of 2 N sulfuric acid. |
Store the unopened kit at 2-8 ℃. Do not use past kit expiration dates.
The kit contains sufficient materials to run an ELISA on one 96-well plate.
Related Product
- Human IL-1ß ELISA (TBS3219)
- Human IL-2 ELISA (TBS3220)
- Human IL-4 ELISA (TBS3221)
- Human IL-6 ELISA (TBS3223)
- Human IL-7 ELISA (TBS3224)
Additional information
SIZE | 1 x 96-well plate |
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