Human Gamma-H2AX/rH2AX Fast ELISA

Description

Tribioscience’s Fast Human γ-H2AX ELISA is designed to quantitatively detect human γ-H2AX levels in serum, plasma, and other biological samples. The main feature is that the kit uses our novel proprietary approaches to combine samples and detections into a one-step instead of the complicated traditional methods. It makes the assay simple, easy, accurate, and fast. The Hands-on time can be within 2 hours, with no need for 4-5 hours (Fig. 1). The detection range is from 312.5 to 20000 pg/mL. The levels of human γ-H2AX samples are parallel to the standard curves obtained using the kit standards linearly. Therefore, the kit can be used to determine relative mass values for natural human γ-H2AX protein.

 

Typical Data

This standard curve (R²=1.000) is provided for demonstration only. A standard curve should be generated for each set of samples assayed. Fig. 3 is an example of typical Data.

Sensitivity

The minimum detectable dose (MOD) of human is typically 10 pg/ml.

The Intra-assay CV and the Inter-assay CV are ˂10%.

Specificity

This assay specifically recognizes Histone H2AX phosphorylated at S139.

No cross-reactivity with unphosphorylated recombinant human (rh) Histone H2AX, rhHistone Macro H2A1, and rhHistone Macro H2A2.

 

Simple Procedures (Fig.1)

 

Simple Standard Preparation

 

Accurate Standard Curve

 

 

Kit Content and Storage Conditions

Part	Part#	Description	Storage of Opened/Reconstituted
Human γ-H2AX Microplate	TBS3265A	96-well polystyrene microplate (12 strips of 8 wells) coated with a monoclonal antibody specific for human γ-H2AX.	Return unused wells to the foil pouch. Reseal along the entire edge of the zip-seal. May be stored for up to 1 month at 2-8 °C.
Human γ-H2AX Standard	TBS3265B	50 µl of Recombinant human γ-H2AX (200 ng/mL).	Aliquot and store at -20 ℃ for up to 1 month in a manual defrost freezer. Avoid repeated freeze-thaw cycles.
Detection A	TBS3265C	2.1 ml of biotin- human γ-H2AX antibody.	May be stored for up to 3 months at 2-8 °C.
Detection B	TBS3265D	12 ml of streptavidin HRP.
Assay Diluent	TBS3265E	12 mL of a buffered protein base with preservatives.
10x Wash Buffer	TBS3000W	12 mL of concentrated solution (10x).
TMB Substrate	TBS3000T	12 mL of ultra-sensitive TMB substrate.
Stop Solution	TBS3000S	6 mL of 2 N sulfuric acid.

Store the unopened kit at 2-8 ℃. Do not use past kit expiration dates.
The kit contains sufficient materials to run an ELISA on one 96-well plate.

 

Related Product

1. Human Total H2AX ELISA (TBS3266)
2. Fast Human Phospho Tau Thr217 (pT217) ELISA (TBS3293)
3. Human p-Tau-181 ELISA (TBS3294)
4. Fast Human MAPT/Tau (total) ELISA (TBS3295)
5. Fast Human Phospho Tau Thr231 (p-T231) ELISA (TBS3296)

Additional information

SIZE	1 x 96-well plate

Datasheet:

TBS3265_human-rH2AX-elisa_datasheet-V01-2022

MSDS:

TBS3265_Human_γ-H2AX_ELISA_MSDS