Human IL-1 α ELISA; 1 step and 1hr (TBS3202)
$420.00
| SKU | Stock | SIZE (kit) | Price | Quantity | ||
|---|---|---|---|---|---|---|
| TBS3202 | Yes | 1 x 96-Well Plate | $420.00 |
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Description
The Fast Human IL-1α ELISA is a solid phase ELISA designed to measure human IL-1α levels in cell culture supernatants, serum, and plasma. The main feature is that the kit uses our novel proprietary approaches to combine samples and detections into a one-step instead of the complicated traditional methods. It makes the assay simple, easy, accurate and fast. The measurement can be finished in 1 hour, with no need for 4-5 hours (Fig. 1). The detection range is from 4 to 1000 pg/mL. The levels of human IL-1α samples are parallel to the standard curves obtained using the kit standards linearly. These results indicate that this kit can be used to determine relative mass values for natural human IL-1α protein.
Typical Data
This standard curve(R2=0.9925) is provided for demonstration only. A standard curve should be generated for each set of samples assayed. Fig. 3 is an example of typical Data.
Sensitivity
The minimum detectable dose (MOD) of human IL-1α is typically 3.0 pg/ml.
The Intra-assay CV is 4.9% the Inter-assay CV is 8.1%.
Specificity
This assay recognizes natural and recombinant human IL-1α.
No cross-reactivity: human IL-1β; Mouse IL-1α; Mouse IL-1β; Rat human IL-1α; porcine IL-1α; porcine IL-1β.
Simple Procedures
Simple Standard Preparation
Accurate Standard Curve
Kit Content and Storage Conditions
| Part | Part# | Description | Storage of Opened/Reconstituted |
|---|---|---|---|
| Human IL-1α Microplate | TBS3202A | 96 well microplate (12 strips of 8 wells) coated with a Capture Antibody specific for human IL-1α. | Return unused wells to the foil pouch. Reseal along the entire edge of the zip-seal. May be stored for up to 1 month at 2-8 °C. |
| Human IL-1α Standard | TBS3202B | 30ul of Recombinant human IL-1α protein (100ng/mL). | Aliquot and store at -20 ℃ for up to 1 month in a manual defrost freezer. Avoid repeated freeze-thaw cycles. |
| Detection A | TBS3202C | 2.1 ml of human IL-1α antibody. | May be stored for up to 3 months at 2-8 °C. |
| Detection B | TBS3202D | 12 ml of Streptavidin-HRP | |
| Assay Diluent | TBS3202E | 12 mL of a buffered protein base with preservatives. | |
| Wash Buffer | TBS3000W | 12 mL of concentrated solution (10x). | |
| TMB Substrate | TBS3000T | 12 mL of ultra-sensitive TMB substrate. | |
| Stop Solution | TBS3000S | 6 mL of 2 N sulfuric acid. |
Store the unopened kit at 2-8 ℃. Do not use past kit expiration dates.
The kit contains sufficient materials to run an ELISA on one 96 well plate.
Additional information
| SIZE (kit) | 1 x 96-Well Plate |
|---|
Datasheet:
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