Fast Mouse Phospho-Tau Thr231 ELISA (TBS3007)
$560.00
Cell culture Supernate, Plasma/Serum p-tau-231
| SKU | Stock | SIZE | Price | Quantity | ||
|---|---|---|---|---|---|---|
| TBS3007 | Yes | 1 x 96-well plate | $560.00 |
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Description
Tribio Fast Mouse Phospho Tau Thr231 (pT-231) ELISA is used to quantify pT-231 levels in mouse CSF, serum, plasma, and other biological samples. The kit uses a simplified one-step detection method that integrates sample processing and assay steps, making the procedure fast, easy, and accurate. Total hands-on time is within 3 hours (compared to 5–6 hours for traditional methods, Fig. 1). The detection range is 0.3–243 ng/mL, and mouse pT-231 levels show good linearity with the standard curve. Therefore, the kit can be used for quantitative analysis of native mouse pT-231 protein.
Typical Data
This standard curve (R2=1.000) is provided for demonstration only. A standard curve should be generated for each set of samples assayed. Fig. 3 is an example of typical Data.
Sensitivity
The minimum detectable dose (MDD) of mouse is typically 200 pg/ml.
The Intra-assay CV and the Inter-assay CV are ˂10%.
Specificity
This assay recognizes natural and recombinant mouse pTau-231.
No cross-reactivity with others.
Simple Procedures
Simple Standard Preparation
Accurate Standard Curve
Kit Content and Storage Conditions
| PART | PART# | DESCRIPTION | STORAGE OF OPENED/ RECONSTITUTED |
| Mouse PTau-231 Microplate | TBS3007A | 96 well polystyrene microplate (12 strips of 8 wells) coated with a monoclonal antibody specific for mouse pT-231. | Return unused wells to the foil pouch. Reseal along the entire edge of the zip-seal. May be stored for up to 1 month at 2-8 °C. |
| Mouse PTau-231 Standard | TBS3007B | 60 µl of Recombinant mouse pT-231 (2.43 μg/mL). | Aliquot and store at -20 ℃ for up to 1 month in a manual defrost the freezer. Avoid repeated freeze-thaw cycles. |
| Detection A | TBS3007C | 2.1 ml of biotin mouse pT-231 Detection antibody. |
May be stored for up to 3 months at 2-8 °C. |
| Detection B | TBS3007D | 12 ml of Streptavidin-HRP | |
| Assay Diluent | TBS3007E | 12 ml of a buffered protein base with preservatives. | |
| Wash Buffer | TBS3000W | 12 ml of concentrated solution (10x). | |
| TMB Substrate | TBS3000T | 12 ml of ultra-sensitive TMB substrate. | |
| Stop Solution | TBS3000S | 6 ml of 2 N sulfuric acid. |
Store the unopened kit at 2-8 ℃. Do not use past kit expiration dates.
The kit contains sufficient materials to run an ELISA on one 96 well plate.
Research use only.
Additional information
| SIZE | 1 x 96-well plate |
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