Equine IL-1β / IL-1 beta ELISA Kit
$410.00
| SKU | Stock | SIZE | Price | Quantity | ||
|---|---|---|---|---|---|---|
| TBS34007 | Yes | 96-well plate | $410.00 |
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Description
Tribioscience’s Fast Equine IL-1β ELISA is designed to quantitatively detect equine IL-1β levels in different tissues including skin, muscle, neural, serum, and other biological samples. The main feature is that the kit uses our novel proprietary approaches to combine samples and detections into a one-step instead of the complicated traditional methods. It makes the assay simple, easy, accurate, and fast. The measurement can be finished in 2 hours, with no need for 4-5 hours (Fig. 1). The detection range is from 15 to 1000 pg/mL. The levels of equine IL-1β samples are parallel to the standard curves obtained using the kit standards linearly. These results indicate that this kit can be used to determine relative mass values for natural equine IL-1β protein.
Typical Data
This standard curve (R2=0.9998) is provided for demonstration only. A standard curve should be generated for each set of samples assayed. Fig. 3 is an example of typical Data.
Sensitivity
The minimum detectable dose (MDD) of equine IL-1β is typically 10 pg/ml.
The Intra-assay CV is 3.79% the Inter-assay CV is ˂10%.
Specificity
This assay recognizes natural and recombinant equine IL-1β.
Simple Procedures (Fig. 1)
Simple Standard Preparation
Accurate Standard Curve
Kit Content and Storage Conditions
| PART | PART# | DESCRIPTION | STORAGE OF OPENED/ RECONSTITUTED |
| Equine IL-1β Microplate | TBS34007A | 96 well polystyrene microplate (12 strips of 8 wells) coated with a polyclonal antibody specific for equine IL-1β. | Return unused wells to the foil pouch. Reseal along entire edge of the zip-seal. May be stored for up to 1 month at 2-8 °C. |
| Equine IL-1β Standard | TBS34007B | 20 µL of Recombinant equine IL-1β protein (50 ng/mL). | Aliquot and store at -20 ℃ for up to 1 month in a manual defrost freezer. Avoid repeated freeze-thaw cycles. |
| Detection A | TBS34007C | 2.2 mL of Biotin-equine IL-1β antibody. |
May be stored for up to 3 months at 2-8 °C.* |
| Detection B | TBS34007D | 200 µL of Streptavidin-HRP. | |
| Assay Diluent | TBS34007E | 25 mL of a buffered protein base with preservatives. | |
| Wash Buffer | TBS3000W | 12 mL of concentrated solution (10x). | |
| TMB Substrate | TBS3000T | 12 mL of ultra-sensitive TMB substrate. | |
| Stop Solution | TBS3000S | 6 mL of 2 N sulfuric acid. |
Store the unopened kit at 2-8 ℃. Do not use past kit expiration dates.
The kit contains sufficient materials to run an ELISA on one 96-well plate.
Research use only.
Additional information
| SIZE | 96-well plate |
|---|
Datasheet:
TBS34007_Equine IL-1 beta ELISA_datasheet_2024
MSDS:
TBS34007_Equine_IL-1β_ELISA_MSDS
Research use only.
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