WST-8 or Cell Count Kit-8 (CCK-8)

WST-8 or Cell Count Kit-8 (CCK-8)

$119.00$928.00

SKU: TBS2022 Categories: ,
SKUStock SIZE (test) PriceQuantity
TBS2022-500Yes500 tests $119.00
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TBS2022-1KYes1000 tests $198.00
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TBS2022-5KYes5000 tests $928.00
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Description

TribioScience’s Cell Counting Kit-8 (CCK-8) is a colorimetric assay to quantify the viable cells, consisting of the tetrazolium salt and the electron mediator 1-mthoxyPMS. The working principle is based on dehydrogenase activity detection. The generated NADH from the reaction of dehydrogenase enzymes and the substrates oxidizes the 1-methoxyPM. The reduced 1-methoxyPMS further oxidizes the tetrazolium salt (WST-8) in the CCK-8 assay kit, forming the yellow formazan dye. The dye color can then be quantified by measuring the absorbance at 460 nm wavelength so that the cell number is known. The assay can be used for the measurement of cell proliferation in response to growth factors, cytokines, mitogens, and nutrients, etc. It can also be used for the analysis of cytotoxic compounds like anticancer drugs and many other toxic agents and pharmaceutical compounds.

 

FEATURES

  • Simplicity: One-bottle, ready-to-use solution. No harvesting, no washing, and no solubilization steps.
  • Safety: No organic solvents or isotopes required.
  • Accuracy: Absorbance measurement is proportional to the number of cells in each well.
  • Sensitivity: More sensitive than MTT, XTT, MTS, or WST-1.

APPLICATIONS

  • Cell proliferation
  • Drug screening
  • Growth factors
  • Cytotoxicity
  • Apoptosis

RELATED PRODUCTS

  1.  Resazurin Cell Viability Kit (TBS2001)
  2.  LDH Cytotoxicity Assay (TBS2002)
  3.  MTT Cell Viability Assay (TBS2003)
  4.  MTS Cell Viability Assay (TBS2004)
  5.  Catalase Assay (TBS2006)

Additional information

SIZE (test)

1000 tests, 500 tests, 5000 tests

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