WST-8 or Cell Count Kit-8 (CCK-8)
$119.00 – $928.00
| SKU | Stock | SIZE (test) | Price | Quantity | ||
|---|---|---|---|---|---|---|
| TBS2022-500 | Yes | 500 Tests | $119.00 | |||
| TBS2022-1K | Yes | 1000 Tests | $198.00 | |||
| TBS2022-5K | Yes | 5000 Tests | $928.00 |
- Description
- Terms
- Additional information
- Documents
- Reviews (1)
Description
TribioScience’s Cell Counting Kit-8 (CCK-8) is a colorimetric assay to quantify the viable cells, consisting of the tetrazolium salt and the electron mediator 1-mthoxyPMS. The working principle is based on dehydrogenase activity detection. The generated NADH from the reaction of dehydrogenase enzymes and the substrates oxidizes the 1-methoxyPM. The reduced 1-methoxyPMS further oxidizes the tetrazolium salt (WST-8) in the CCK-8 assay kit, forming the yellow formazan dye. The dye color can then be quantified by measuring the absorbance at 460 nm wavelength so that the cell number is known. The assay can be used for the measurement of cell proliferation in response to growth factors, cytokines, mitogens, nutrients, etc. It can also be used for the analysis of cytotoxic compounds like anticancer drugs and many other toxic agents and pharmaceutical compounds.
Principle
Features
- Simplicity: One-bottle, ready-to-use solution. No harvesting, no washing, and no solubilization steps.
- Safety: No organic solvents or isotopes are required.
- Accuracy: Absorbance measurement is proportional to the number of cells in each well.
- Sensitivity: More sensitive than MTT, XTT, MTS, or WST-1.
Applications
- Cell proliferation
- Drug screening
- Growth factors
- Cytotoxicity
- Apoptosis
CCK-8 Performance for Cell Viability displayed in Fig.1-3
Additional information
| SIZE (test) | 1000 Tests, 500 Tests, 5000 Tests |
|---|
chenzane –
We used Cell Counting Kit 8 of Tribioscience recently. Comparing the specificity and sensitivity of it to that of MTT using the Hela cell line and MCF-7 cell line that were treated by different anti-canacer antibodies. We found that It was more sensitive than the MTT assay in both cell lines, especially after incubation for 2hs. We recommend CCK8 instead of MTT if there is problems due to lymphocytes. It is worthy to try.