Glutamic Oxaloacetic Transaminase (GOT)/ Aspartate aminotransferase (AST) Activity Colorimetric Assay (TBS2013)

Description

Aspartate Aminotransferase (EC2.6.1.1, AST), also known as glutamic-oxaloacetic transaminase (GOT), is an enzyme that catalyzes the reversible transfer of an amino group from aspartate. This reaction is a key part of the nitrogen metabolism and the citric acid cycle, connecting amino acid metabolism to energy production. AST is found in various tissues, particularly in liver (high concentration), heart (cardiac muscle), skeletal muscle, kidneys and brain. Cytoplasmic AST and mitochondrial AST are found in circulation. When elevated or reduced in the blood, it can indicate metabolic disorders. So it is a key marker in diagnosing or monitoring these conditions.

Tribio™ Aspartate Aminotransferase Activity Colorimetric Assay is based on a dehydrogenase coupled reaction converting chromophore to a colored formazan, which can be measured at OD 460 nm. The generated signal is proportional to the AST enzyme activity. The kit provides the easiest and most accurate approach to measuring AST activity from a variety of samples.

Applications

Specifically detect aspartate aminotransferase from cells and biological tissues.

Kit Contents for 100 tests:

Storage conditions: Store the Reagent at –20 ℃ for a year.

Typical standard curve

The Typical Standard curve showed as an example in Fig.1. It can be used only for reference.

The positive control graph is shown in Fig.2 as below.

Research use only.

Additional information

Datasheet:

TBS2013_Aspartate Aminotransferase Colorimetric Assay_data_sheet_2026

MSDS:

TBS2013-GOT AST Activity Colorimetric Assay_MSDS_2024

Research use only.