Fast Glucose Oxidase Activity Assay (Colorimetric/Fluorometric)

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Fast Glucose Oxidase Activity Assay (Colorimetric/Fluorometric)

$269.00$660.00

Tribioscience’s Fast Glucose Oxidase Acitivty Assay uses a single working reagent that combines the glucose oxidase reaction and color reaction in one step.

SKU: TBS2088 Category: Tag:
SKUStock SIZE PriceQuantity
TBS2088-200Yes200 Assays $269.00
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TBS2088-600Yes600 Assays $660.00
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Description

Glucose oxidase (GOD) catalyzes the oxidation of glucose from D-glucose to D-glucono-δ-lactone. Physilogically, it aids in the breakdown of glucose into smaller metabolites. It is widely used in electrochemical glucose sensons designed for diabetes patients. Simple, direct, and high-throughput assays for measuring glucose oxidase activity find wide applications in research and drug discovery.
Tribioscience’s Fast Glucose Oxidase Acitivty Assay uses a single working reagent that combines the glucose oxidase reaction and color reaction in one step. The change in color intensity of the reaction product at 570nm or fluorescence intensity at λ ex/em = 530/585 nm is directly proportional to glucose oxidase activity in the sample. The fluorometric assay is more sensitive than the colorimetric assay.

Applications:

Direct Assay: Glucose oxidase activity in serum, plasma, urine, and other bio-samples.

Key Features:

Flexible: Suitable for colorimetric and fluorometric methods.
Accurate: Use 50μL samples. Detection ranges 0.1-50 μM in 96-well plate for colorimetric assay and 0.002-10 μM for fluorometric assay.
Simple and High-Throughput: One-step procedure. Load-Incubate-Read. Kit can be used for a robust method.
Time Saving: takes less than 30 minutes.

Kit Contents:

ComponentTBS2088-200TBS2088-600
Assay Buffer1x 12mL3x 12mL
Red Probe1x 0.25mL1x 0.8mL
GOD Standard Stock (1 U/mL)1x 25μL1x 80μL
Enzymes1x 2mL3x 2mL

 

Storage and Handling:

Store kit at -20℃.
Shelf life of 6-months.
Protect from light.

Example Fluorometric Standard Curve:
Image of Example Fluorometric Standard Curve

Example Colorimetric Standard Curve:
Image of Example Colorimetric Standard Curve

Additional information

SIZE

200 Assays, 600 Assays

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