Beta-N-Acetylglucosaminidase (Beta-NAG) Activity Colorimetric Assay
$290.00 – $490.00
SKU | Stock | SIZE (test) | Price | Quantity | ||
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TBS2105-100 | Yes | 100 Assays | $290.00 | |||
TBS2105-200 | Yes | 200 Assays | $490.00 |
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Description
β-Hexosaminidase (β-N-Acetylglucosaminidase, NAGase, EC 3.2.1.52) is a lysosomal enzyme that is expressed in various tissues, including kidneys, liver, lungs, and mast cells. Elevated enzyme levels are associated with many disorders, such as Tay-Sachs and Sandhoff disease, inflammation, and abnormal immune responses.
The Beta-Hexosaminidase Activity Colorimetric Assay provides a simple and sensitive method for monitoring hexosaminidase activity in biological samples (tissue, cells, serum, urine). This assay uses a synthetic p-nitrophenol derivative (R-pNP) as its substrate and releases pNP which can be measured at absorbance (OD 405 nm). The assay can detect as low as 50 µU of NAGase activity in a variety of samples.
Applications
This kit is used for the determination of hexosaminidase activity in biological samples.
Key features
- Fast and sensitive: Linear detection range (20 µL sample): 0.05 to 50 U/L for a 30-minute reaction at 37˚C.
- High throughput: This can be readily automated on HTS liquid handling systems for processing thousands of samples per day.
Storage Conditions
This kit is shipped on ice. Store all components at -20℃ in a non-frost-free freezer. Shelf life of 12 months after receiving.
Preparation
Standard Curve
Additional information
SIZE (test) | 100 Assays, 200 Assays |
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