Hot

Beta-N-Acetylglucosaminidase (Beta-NAG) Activity Colorimetric Assay

$290.00$490.00

SKU: TBS2105-NAG Category:
SKUStock SIZE (test) PriceQuantity
TBS2105-100 Yes 100 Assays $290.00
spinner
TBS2105-200 Yes 200 Assays $490.00
spinner

Description

β-Hexosaminidase (β-N-Acetylglucosaminidase, NAGase, EC 3.2.1.52) is a lysosomal enzyme that is expressed in various tissues, including kidneys, liver, lungs, and mast cells. Elevated enzyme levels are associated with many disorders, such as Tay-Sachs and Sandhoff disease, inflammation, and abnormal immune responses.

The Beta-Hexosaminidase Activity Colorimetric Assay provides a simple and sensitive method for monitoring hexosaminidase activity in biological samples (tissue, cells, serum, urine). This assay uses a synthetic p-nitrophenol derivative (R-pNP) as its substrate and releases pNP which can be measured at absorbance (OD 405 nm). The assay can detect as low as 50 µU of NAGase activity in a variety of samples.

 

Applications

This kit is used for the determination of hexosaminidase activity in biological samples.

 

Key features

  • Fast and sensitive: Linear detection range (20 µL sample): 0.05 to 50 U/L for a 30-minute reaction at 37˚C.
  • High throughput: This can be readily automated on HTS liquid handling systems for processing thousands of samples per day.

Storage Conditions

This kit is shipped on ice. Store all components at -20℃ in a non-frost-free freezer. Shelf life of 12 months after receiving.

 

Preparation

 

Standard Curve

 

Additional information

SIZE (test)

100 Assays, 200 Assays

Reviews

There are no reviews yet.


Be the first to review “Beta-N-Acetylglucosaminidase (Beta-NAG) Activity Colorimetric Assay”