Beta-Hexosaminidase Activity Colorimetric Assay (TBS2105)
$290.00
SKU | Stock | SIZE (test) | Price | Quantity | ||
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TBS2105 | Yes | 100 Assays | $290.00 |
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Description
β-Hexosaminidase are hydrolytic enzyme responsible for the breakdown of carbohydrates. Specifically, β-Hexosaminidase cleaves the terminal β-D-glucuronic acid residue from the nonreducing terminus of a mucopolysaccharide chain. In humans, these enzymes are found in the lysosome of many tissue types. Loss of β-Hexosaminidase activity results in metabolic disease and health problems. It is important to detect β-Hexosaminidase activity in the tested samples for disease examination.
The Beta-Hexosaminidase Activity Colorimetric Assay provides a simple and sensitive method for monitoring hexosaminidase activity in biological samples (tissue, cells, serum, urine). This assay uses a synthetic p-nitrophenol derivative (R-pNP) as its substrate and releases pNP which can be measured at absorbance (OD 405 nm). The assay can detect as low as 50 µU of NAGase activity in a variety of samples.
Applications
This kit is used for determination of β-Hexosaminidase activity in biological samples.
Key features
- Fast and sensitive: Linear detection range (20 µL sample): 0.05 to 50 U/L for a 30 minutes reaction at 37˚C
- High throughput: This can be readily automated on HTS liquid handling systems for processing thousands of samples per day.
Storage Conditions
The kit is shipped on ice and should be stored at -20°C for long-term storage. Shelf life of 12 months after receipt.
Preparation
Standard Curve
Additional information
SIZE (test) | 100 Assays, 200 Assays |
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