Beta-Hexosaminidase Activity Colorimetric Assay (TBS2105)

Description

β-Hexosaminidase are hydrolytic enzyme responsible for the breakdown of carbohydrates. Specifically, β-Hexosaminidase cleaves the terminal β-D-glucuronic acid residue from the nonreducing terminus of a mucopolysaccharide chain. In humans, these enzymes are found in the lysosome of many tissue types. Loss of β-Hexosaminidase activity results in metabolic disease and health problems. It is important to detect β-Hexosaminidase activity in the tested samples for disease examination.

The Beta-Hexosaminidase Activity Colorimetric Assay provides a simple and sensitive method for monitoring hexosaminidase activity in biological samples (tissue, cells, serum, urine). This assay uses a synthetic p-nitrophenol derivative (R-pNP) as its substrate and releases pNP which can be measured at absorbance (OD 405 nm). The assay can detect as low as 50 µU of NAGase activity in a variety of samples.

Applications

This kit is used for determination of β-Hexosaminidase activity in biological samples.

 

Key features

• Fast and sensitive: Linear detection range (20 µL sample): 0.05 to 50 U/L for a 30 minutes reaction at 37˚C
• High throughput: This can be readily automated on HTS liquid handling systems for processing thousands of samples per day.

Storage Conditions

The kit is shipped on ice and should be stored at -20°C for long-term storage. Shelf life of 12 months after receipt.

 

Preparation

 

Standard Curve

 

Additional information

SIZE (test)	100 Assays, 200 Assays

Datasheet:

TBS2105-β-Hexosaminidase Activity Assay-2022

MSDS:

TBS2105 Beta Hexosaminidase Assay SDS