Pfu PCR MasterMix (2x) (TBS4012)

Description

Pfu PCR MasterMix is an optimized 2 x premix reagent composed of Super Pfu DNA Polymerase, Mg2+, dNTPs, and reaction buffer. Super Pfu DNA polymerase has 3 ´ →5 ´ exonuclease activity, and its fidelity is about 100 times higher than Taq DNA polymerase, making it an ideal choice for cloning. This enzyme achieves high-speed PCR with an extension rate of 10 sec/kb while maintaining high fidelity through the introduction of extension enhancement technology. The amplification length can reach 20 kb. In addition, this product can amplify long fragments, GC rich regions, and excess templates that are difficult to amplify with high success rate. The PCR product obtained by amplification does not contain the “A” base at the 3 ‘end and can be directly cloned into a blunt ended vector.

Applications

This kit is used for PCR amplification.

Key Features

• High-fidelity: The fidelity is about 100 times higher than Taq DNA polymerase. Long target fragments can also be amplified rapidly and with high fidelity.
• High-speed: When amplifying target fragments below 1kb, the extension time can be set to 10 seconds. When amplifying the target fragment of 1-10 kb, the extension time can be set to 20-30 sec/kb.
• Simple and convenient: This reagent contains all PCR components except primers and templates, which facilitates operation and improves the reproducibility of the results.

Kit Contents

Storage Conditions

Store all components at -20°C in a non-frost-free freezer.

Shelf life is 12 months after receipt.

The kit is shipped on ice.

Research use only.

Additional information

Datasheet:

TBS4012-2xPfu-PCR_master mix

MSDS:

TBS4012_Pfu PCR MasterMix (2x)_MSDS

Research use only.