Mycoplasma Titer qPCR (TBS42030C)

Description

Mycoplasma Titer qPCR is designed to quantitatively detect the mycoplasma levels in test samples using real-time quantitative polymerase chain reaction (qPCR) and probe fluorescence labels. It provides a sensitive, accurate and simple method to titer mycoplasma concentration in testing samples (See Fig.1, and Table 4).

Applications:

Detect mycoplasma-target DNA in contaminated cell cultures, FBS medium, plant, cannabis, cannabis ingredients, grain, food, herbals, and animal feed.

Key Features:

• Quantitate mycoplasma concentration.
• High sensitivity and specificity for mycoplasma species.
• High efficiency: the optimal systemic conditions for PCR amplifications.
• Streamlined protocol: Just add DNA Template and water.

Storage Conditions:

The kit is shipped on ice and stored at -20°C for long-term storage. Shelf life of 12 months after receipt.

Fig.1. Mycoplasma DNA concentration dependent qPCR Amplification (FAM)

Fig.2 Internal Control Gene qPCR Amplification (Hex)

Fig.3. Mycoplasma DNA preparation method validation

Table 1: Mycoplasma DNA qPCR Sensitivity

Table 2: Mycoplasma DNA efficiency comparison from 3 methods

Research use only.

Additional information

Data Sheet:

TBS42030C_Mycoplasma_Titer qPCR kit_V06

MSDS:

TBS42030C_Mycoplasma Titer qPCR_MSDS

Research use only.