Mycoplasma Detection qPCR Kit

$239.00$476.00

The Tribioscience Mycoplasma Detection qPCR Kit is designed to specifically detect potential Mycoplasma contamination in cell cultures with probe-based real-time PCR technology.

SKU: TBS42030 Category:
SKUStock SIZE PriceQuantity
TBS42030-100 Yes 100 Reactions $239.00
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TBS42030-200 Yes 200 Reactions $476.00
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Description

Mycoplasma Species qPCR Kit has been designed to specifically identify the mycoplasma genus species in a single PCR reaction using real-time quantitative polymerase chain reaction (qPCR) and probe fluorescence labels. The detection of the target DNA confirms ingredient authenticity and prevents food fraud, ethical issues, or health concerns. Tribioscience’s Mycoplasma qPCR is very sensitive, accurate, and High efficiency (See Fig.1 and Table 1).

 

Applications:

Detect Mycoplasma target DNA in contaminated cell cultures, FBS medium, plant, cannabis, cannabis ingredients, grain, food, herbals, and animal feed.

Key Features:

  • High sensitivity and specificity for Mycoplasma species.
  • High efficiency: the optimal systemic conditions for PCR amplifications.
  • Streamlined protocol: Just add DNA Template and water.
  • No cross reactivity with other species

 

Storage Conditions:

The kit is shipped on ice. Store all components at -20°C in a non-frost-free freezer. Shelf life is 12 months after receiving.

 

Fig.1. Mycoplasma DNA concentration dependent qPCR Amplification (FAM)

Fig.2 Internal Control Gene qPCR Amplification (Hex)

 

Fig.3. Mycoplasma DNA preparation method validation

 

Table 1: Mycoplasma DNA qPCR Sensitivity

 

Table 2: Mycoplasma DNA efficiency comparison from 3 methods

Related Products

  1. TBS6025: Microbial DNA Magnetic Extraction
  2. TBS 42020: Universal Aspergillus qPCR
  3. TBS42021: Aspergillus Flavus qPCR
  4. TBS42022: Aspergillus Fumigatus qPCR
  5. TBS42023: Aspergillus Niger qPCR

Additional information

SIZE

200 Reactions, 100 Reactions

3 reviews for Mycoplasma Detection qPCR Kit

  1. 5 out of 5

    jchong

    DNA extraction protocol was simple and efficient, only requiring enrichment and boiling steps to achieve sufficient amounts of Mycoplasma DNA. The other kit components for RT-qPCR also worked well with no errors.

  2. 5 out of 5

    JYT

    User friendly – highly recommended!

  3. 5 out of 5

    jianxinh

    Very good products. the data is very precise. manual is written clear.


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