Clostridium Botulinum Detection Kit (TBS42063)

$360.00

SKU: TBS42063 Category:
SKUStock SIZE (test) PriceQuantity
TBS42063-100 Yes 100 Tests $360.00
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Description

Clostridium botulinum is a species of gram-positive, anaerobic, spore-forming bacterium. It is commonly found in soil, water, and the gastrointestinal tract of humans and animals. Its detection is critical for quality control, contamination monitoring, and scientific research. Tribioscience Clostridium botulinum qPCR Detection Kit is specifically used to detect Clostridium botulinum species in a single real-time quantitative PCR (qPCR) reaction using fluorescent probes. Detection of the target DNA confirms ingredient authenticity and helps prevent food fraud to ensure food safety.

PRINCIPLE

The kit is based on probe real-time qPCR technology, which amplifies specific gene sequence of Clostridium botulinum. Target-specific fluorescent probes bind to the amplified PCR products. As the products accumulate, the fluorescence signal increases in PCR amplification. By monitoring fluorescence intensity in real time, the kit allows the quantitative detection of the target DNA during the PCR run and provides rapid and accurate results.

KEY FEATURES

• High sensitivity for Clostridium botulinum.
• High efficiency: the optimal systemic conditions for PCR amplifications.
• Streamlined protocol: just add DNA Template and water.
• No cross reactivity with other species.

APPLICATIONS

qPCR detection of Clostridium botulinum is used in food and environmental, fermentation process control, spoilage prevention, and microbial ecology studies to rapidly and specifically quantify the bacteria.

KIT CONTENTS

Name 100x rxn
qPCR Super Mix (B1) 0.9 mL
Primer-probe Mix (B2) 0.5 mL
Positive Control DNA (B+) 20 µL
Negative Control DNA (B) 60 µL

The Clostridium botulinum target probe has been labeled with FAM while the PCR internal control has been labeled with Hex.

STORAGE CONDITION

The kit is shipped on ice and stored at -20°C for long-term storage. Shelf life of 12 months after receipt.

 

Fig.2: DNA Standard Curve

Fig.3: DNA Standard Amplification

Research use only.

Additional information

SIZE (test)

100 Tests

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