Long Flash PCR Master Mix (TBS4044)

Description

The Long Flash PCR Master Mix is a convenient, high-performance solution that includes Super DNA Polymerase, dNTPs, unique elongation factors, and an optimized buffer system, allowing users to perform PCR by simply adding primers and template DNA. The Super DNA Polymerase features both 5′→3′ polymerase and 3′→5′ exonuclease (proofreading) activity, with a fidelity approximately 80 times higher than that of Taq polymerase and generates blunt-end products. It’s hot-start capability ensures the enzyme remains inactive at temperatures ≤55°C and becomes fully active after a 30-second incubation at 98°C. This mix is particularly suitable for amplifying DNA fragments from 100 bp up to 40 kb, with excellent performance for fragments ≥10 kb. With a fast extension rate of 10 seconds per kilobase, it significantly shortens PCR reaction time. Offering high fidelity, strong amplification efficiency, and resistance to inhibitors, the Long Flash PCR Master Mix is well-suited for downstream applications such as molecular cloning and first-, second-, and third-generation sequencing.

Components

Features

• Long fragments: The human genomic DNA template can amplify a 34kb target fragment.
• Ultra-fast: The extension time can be as fast as 1 sec/kb (target fragment within 2 kb)
• High GC: The human genomic DNA template can amplify 40% to 80% GC content.
• High resistance to adversity: Effectively amplify samples such as blood and mouse tail crude extract.

Shipping and Storage:

-20°C.

Research use only.

Additional information

Datasheet:

TBS4044_Long Flash PCR Master Mix_datasheet_v03

MSDS:

TBS4044_Long Flash PCR Master Mix_MSDS

Research use only.