Sarcosine oxidase, Enzyme Activity (TBP0042)

$178.00$712.00

SKU: TBP0042 Category:
SKUStock SIZE PriceQuantity
TBP0042-100U Yes 100 U $178.00
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TBP0042-500U Yes 500 U $712.00
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Description

Preparation and Specification

Appearance: Yellowish amorphous powder, lyophilized

Activity: GradeⅢ 8.0U/mg-solid or more

Contaminant: Catalase ≤1.0%

Stabilizers: Potassium gluconate

 

Properties

Stability: Stable at -20°C for at least One year

Molecular weight: approx.43,000 (by SDS-PAGE)

Isoelectric point: 4.8±0.1

Michaelis constant: 2.8×10⁻³M

Inhibitors: Cu⁺⁺, Ag⁺, Hg⁺⁺, p-chloromercuribenzoate, N-ethylmaleimide, SDS

Optimum pH: 7.5-8.5

Optimum temperature: 55-60°C

pH Stability: 6.0-9.5 (25°C, 20hr)

Thermal stability: below 60°C (pH 7.5, 30min)

 

Applications

This enzyme is useful for enzymatic determination of creatinine, creatine, and sarcosine when coupled with creatinine amidohydrolase (CNH-211, CNH-311) and creatine amidinohydrolase (CRH-211, CRH- 221, CRH-229).

 

Additional information

SIZE

100 U, 500 U

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