Purine-nucleoside phosphorylase, Enzyme Activity (TBP0038)

Description

Preparation and Specification

Appearance: White amorphous powder, lyophilized

Activity: GradeⅢ 15U/mg-solid or more

Contaminants: Catalase ≤20%

5′-Nucleosidase ≤1.0×10⁻³%

Adenosine deaminase ≤1.0×10⁻³%

ATPase ≤1.0×10⁻²%

Stabilizers: K-Gluconate, mannitol, EDTA

 

Properties

Stability: Stable at -20°C for at least One year

Molecular weight: approx. 120,000

Isoelectric point: 4.1±0.1

Michaelis constants: 6.4×10⁻⁵M (Inosine), 3.2×10⁻⁴M (Pi)

Inhibitors: p-Chloromercuribenzoate, SDS, Hg⁺⁺, Ag⁺

Optimum pH: 7.5-8.0

Optimum temperature: 65°C

pH Stability: pH 6.0-9.0 (30°C, 16hr)

Thermal stability: below 60°C (pH 7.7, 30min)

 

Applications

This enzyme is useful for enzymatic determination of inorganic phosphorus, 5′-nucleotidase and adenosine deaminase when coupled with xanthine oxidase (XTO-212) and uricase (UAO-201, UAO- 211)

Additional information

SIZE (mg)	1 MG, 5 MG

Datasheet:

TBP0038-Purine-nucleoside phosphorylase

MSDS:

TBP0038_Purine-nucleoside phosphorylase Safety Data Sheet