Peroxidase, Enzyme Activity (TBP0037)

Description

Preparation and Specification

Appearance: Reddish-brown amorphous powder, lyophilized

Activity: GradeⅠ 250 Purpurogallin U/mg-solid or more (-131) (RZ ≥ 3.0, salt free)

GradeⅢ 110 Purpurogallin U/mg-solid or more (-301) (RZ ≥ 2.0, containing approx. 30% of stabilizers)

GradeⅢ 180 Purpurogallin U/mg-solid or more (-302) (RZ ≥ 2.0, salt free)

Contaminant: Phosphatase ≤1.0×10⁻³% (GradeⅢ)

Properties

Stability: Stable at -20°C for at least Two years

Molecular weight: approx. 40,000

Structure: Glycoprotein with one mole of protohaemin IX

Inhibitors: Cyanide, sulfide, fluoride, azide

Optimum pH: 6.0-7.0

Optimum temperature: 45°C

pH Stability: pH 5.0-10.0 (25°C, 20hr)

Thermal stability: below 50°C (pH 6.0, 10min)

Applications

This enzyme is useful for enzymatic determination of H₂O₂ in clinical analysis. Especially, the highly purified preparation (Grade I) is useful as a protein tracer in histo-and cyto-chemistry and as a valuable experimental tool in hodological neurography. Also, the enzyme preparation has been used as an enzyme label in enzyme immunoassay. Grade III is suitable for dry chemistry. On the other hand, the enzymes contribute for the reduction of phehol in waste water.

Research use only.

Additional information

Datasheet:

TBP0037-Peroxidase

MSDS:

TBP0037_Peroxidase Safety Data Sheet

Research use only.