Poly(A) Polymerase, Yeast (TBP0178)
$54.00
| SKU | Stock | SIZE (ul) | Price | Quantity | ||
|---|---|---|---|---|---|---|
| TBP0178 | Yes | 100 uL | $54.00 |
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Description
Poly(A) Polymerase catalyses the template independent addition of adenosine residues onto the 3’ ends of polyribonucleotides. The use of ATP as a substrate leads to poly(A) tailing whereas substitution of cordycepin-5’-triphosphate (3’- dATP) for ATP results in addition of a single dA residue to the 3’-termini of the RNA. Neither ADP nor dATP can be used as substrates for this enzyme. Poly(A) Polymerase from yeast has been shown to be more effective at oligonucleotide-labeling and poly(A) tailing of long RNA templates than Poly(A) Polymerase from E. coli.
Component
| Product Component | Quantity |
| Poly(A) Polymerase, Yeast (1 U/µl) | 100 µl |
| 5X Poly(A) Polymerase, Yeast Reaction Buffer | 1 ml |
| 25 mM MnCl₂ | 500 µl |
| ATP (10 mM) | 150 µl |
Store at -20°C.
Product Applications
- Labelling of RNA with ATP or cordycepin
- Poly(A) tailing of RNA for cloning or affinity purification
- Increasing translation of RNA transferred into eukaryotic cells
Research use only.
Additional information
| SIZE (ul) | 100 uL |
|---|
Datasheet:
TBP0178-Poly(A) Polymerase, Yeast_datasheet
MSDS:
TBP0178_Poly(A) Polymerase, Yeast_MSDS
Research use only.
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