Phi29 DNA Polymerase (TBP0177)
$157.00
| SKU | Stock | SIZE (ul) | Price | Quantity | ||
|---|---|---|---|---|---|---|
| TBP0177 | Yes | 100 uL | $157.00 |
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Description
Ultra-Pure Phi29 DNA Polymerase is a highly processive polymerase which exhibits a strong strand-displacement function. These functions allow for highly efficient isothermal amplification of circular or linear DNA templates via rolling circle amplification (RCA), multiple displacement amplification (MDA) and/or whole genome amplification (WGA). Ultra-Pure Phi29 DNA Polymerase has extremely high fidelity due to its inherent 3’→5’ exonuclease activity and can amplify from very small amounts of starting templates. The enzyme is subject to a rigorous multi-step purification protocol using physical, chemical, and enzymatic methods for maximum removal of contaminating genomic DNA. Quality control involves a non-specific DNase Activity Assay and qPCR DNA Contamination Test with a TaqMan probe.
Component
| Product Component | Quantity |
| Ultra-Pure Phi29 DNA Polymerase | 100 μl (200 rxn) |
| 5X Ultra-Pure Phi29 DNA Polymerase Reaction Buffer1 | 500 μl |
1Buffer contains 0.5 μg/μl BSA.
Store all at -20°C. Avoid repeated freeze-thaw cycles of all components to retain maximum performance. All components are stable for 1 year from the date of shipping when stored and handled properly
Product Applications
- Rolling circle amplification (RCA)
- Multiple displacement amplification (MDA)
- Whole genome amplification (WGA)
- DNA template preparation for sequencing
- Protein-primed DNA amplification
Research use only.
Additional information
| SIZE (ul) | 100 uL |
|---|
Datasheet:
TBP0177-Phi29 DNA Polymerase_datasheet
MSDS:
TBP0177_Phi29 DNA Polymerase_MSDS
Research use only.
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