TEV Protease (TBP0179)
$49.00
| SKU | Stock | SIZE (ul) | Price | Quantity | ||
|---|---|---|---|---|---|---|
| TBP0179 | Yes | 100 uL | $49.00 |
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Description
TEV Protease is an improved version of the site-specific protease from Tobacco Etch Virus (TEV). TEV Protease has enhanced activity, stability and sitespecificity when compared to the native enzyme. High specificity cleavage occurs between the Gln and Gly (or Ser) of the seven amino acid recognition sequence Glu-Asn-Leu-Tyr-Phe-Gln-Gly/Ser (ENLYFQ(G/S)) in the fusion protein of interest. TEV Protease is active over a wide range of temperatures (4 – 30°C; optimum 30°C) and pHs (5.5 – 9.0). At the optimal cleavage temperature for TEV Protease, 99% cleavage is often achieved in 1-2 hours.
Component
| Product Component | Quantity |
| TEV Protease (10 U/µl) | 100 µl |
| 20X TEV Protease Reaction Buffer | 1 ml |
| 100 mM DTT | 500 µl |
Store at -20°C.
Product Applications
- Cleavage of tags from recombinant fusion proteins containing a TEV recognition site
- One step affinity removal of his-tagged TEV after cleavage
Research use only.
Additional information
| SIZE (ul) | 100 uL |
|---|
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