Peroxidase, Enzyme Activity (TBP0037)

Description

Preparation and Specification

Appearance: Reddish-brown amorphous powder, lyophilized

Activity: GradeⅠ 250 Purpurogallin U/mg-solid or more (-131) (RZ ≥ 3.0, salt free)

GradeⅢ 110 Purpurogallin U/mg-solid or more (-301) (RZ ≥ 2.0, containing approx. 30% of stabilizers)

GradeⅢ 180 Purpurogallin U/mg-solid or more (-302) (RZ ≥ 2.0, salt free)

Contaminant: Phosphatase ≤1.0×10⁻³% (GradeⅢ)

 

Properties

Stability: Stable at -20°C for at least Two years

Molecular weight: approx. 40,000

Structure: Glycoprotein with one mole of protohaemin IX

Inhibitors: Cyanide, sulfide, fluoride, azide

Optimum pH: 6.0-7.0

Optimum temperature: 45°C

pH Stability: pH 5.0-10.0 (25°C, 20hr)

Thermal stability: below 50°C (pH 6.0, 10min)

 

Applications

This enzyme is useful for enzymatic determination of H₂O₂ in clinical analysis. Especially, the highly purified preparation (Grade I) is useful as a protein tracer in histo-and cyto-chemistry and as a valuable experimental tool in hodological neurography. Also, the enzyme preparation has been used as an enzyme label in enzyme immunoassay. Grade III(-302) is suitable for dry chemistry. On the other hand, the enzymes contribute for the reduction of phehol in waste water.

Additional information

SIZE	1000 U, 5000 U

Datasheet:

TBP0037-Peroxidase

MSDS:

TBP0037_Peroxidase Safety Data Sheet