beta-Nicotimamide-Adenine Dinucleotide Phosphate, Reduced, Coenzyme (TBP0097)
$212.00 – $848.00
SKU | Stock | SIZE (mg) | Price | Quantity | ||
---|---|---|---|---|---|---|
TBP0097-100MG | Yes | 100 MG | $212.00 | |||
TBP0097-500MG | Yes | 500 MG | $848.00 |
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Description
Product Details
Form: Crystalline powder
Molecular Weight: 833.4
Solubility: Distilled water or dilute buffer
Stability: Store at -20° C (-4° F)
Purity: 98+%
Catalog No.: 219J0000
Applications
NADPH is used in the determination of leucine aminopeptidase, creatine, ammonia and urea.
Reagents
- 0.1M Triethanolamine buffer/substrate, pH 7.6: 1.86 g TEA&HCl, 210 mg glycerate-3-P, 125 mg MgSO4&7H2O and 50 mg EDTA with 80 ml distilled water. Adjust pH to 7.6 with 1M NaOH-Na2 and adjust volume to 100 ml with distilled water.
- 3 M Ammoniumcholride:.16.5 g NH4Cl in distilled water, adjust volume to 100 ml.
- 0.2 M a-Ketoglutarate: 45 mg a-Ketoglutarate-Na2&2H2O in 1 ml distilled water.
- Glutamate dehydrogenase, from bovine liver (20 mg protein/ml): 120 U/mg.
Procedure
- Dissolve 25 mg NADP in 25 ml distilled water in a volumetric flask.
- Set spectrophotometer (equipped with strip chart recorder and temperature control) at 340 nm and 25°C.
- Into a cuvette, pipette the following:
Buffer (1) 2.50 ml
NH4Cl (2) 0.15 ml
a-KG (3) 0.10 ml
Mix and read the absorbance A1.
- Add 0.10 ml of the sample. Mix and read absorbance A2.
- Start reaction by adding 0.01 ml GLDH. Mix and read absorbance A3.
- Add an additional 0.01 ml GLDH. Mix and read absorbance A4 (absorbance due to enzyme).
Additional information
SIZE (mg) | 500 MG, 100 MG |
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Datasheet:
TBP0097-beta-Nicotimamide-Adenine Dinucleotide Phosphate, Reduced
MSDS:
TBP0097_beta-Nicotimamide-Adenine Dinucleotide Phosphate, Reduced Safety Data Sheet
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