Safe-Green Nucleic Acid Stain (TBS6111)

Description

Safe-Green is a new and safe class of gel loading dye for the visualization of double-stranded DNA, single-stranded DNA, and RNA in agarose gels. The dye was developed to replace Ethidium Bromide (EtBr, a potent mutagen), commonly used in gel electrophoresis for visualization of nucleic acids in agarose gels. Safe-Green is non-carcinogenic by the Ames-test. The results are negative in both the mouse marrow chromophilous erythrocyte micronucleus and mouse spermary spermatocyte chromosomal aberration tests. Safe-Green loading dye does not require the addition of any stain to the molten agarose, simplifying workflow processes. The product is provided in a ready to use 6X format and is added directly to samples prior to loading onto the gel. Safe-Green loading dye eliminates contamination risk of glassware or agarose gel apparatus as associated with EtBr.

Component

Store at 4°C.

Features

Convenient: Safe-Green is provided as a 6X loading dye, and is mixed directly with samples before gel loading. Inert tracking dye is included to monitor gel progress.
Easy to Use: View and document your results as you would with EtBr staining. Safe-Green can be excited with blue or UV light, and has maximum emission at 525 nm.
Safe: Non-carcinogenic.
Sensitive: Detect as little as 0.2 – 0.6 ng of DNA per gel band.
Superior: EtBr is known to cause strand breaks and nicks in DNA. Using Safe-Green minimizes such damage, yielding higher transformation rates and lower mutation rates versus EtBr. For even better cloning results, use Safe-Green with blue light excitation.

Research use only.

Additional information

Datasheet:

TBS6111-Safe-Green_datasheet

MSDS:

TBS6111_Safe-Green_MSDS

Research use only.