Plasmid DNA Rapidprep Mini Kit
$58.00 – $198.00
Tribo™ Plasmid DNA Rapidprep Mini Kit provides a fast, simple, and cost-effective plasmid DNA preparation method for routine molecular biology laboratory applications. This Rapidpep Mini Kits uses glass microfiber membrane and Clear filter to combine the lysate clearance and the plasmid DNA binding into one step.
| SKU | Stock | SIZE | Price | Quantity | ||
|---|---|---|---|---|---|---|
| TBS6011-50 | Yes | 50 Samples | $58.00 | |||
| TBS6011-200 | Yes | 200 Sample | $198.00 |
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Description
Tribo™ Plasmid DNA Rapidprep Mini Kit provides a fast, simple, and cost-effective plasmid DNA preparation method for routine molecular biology laboratory applications. This Rapidprep Mini Kit uses a glass microfiber membrane and clear filter to combine the lysate clearance and the plasmid DNA binding into one step. This combination significantly increases the purity and yields of plasmid and shortens 50% of the processing time in comparison with similar products on the market. The procedure can be finished in 10 minutes. The ideal use of this kit would be to isolate and purify plasmid with less than 10kb. The efficiency may be reduced with the increased size.
Applications
- Isolate and purify plasmid DNA from 1-3 ml of E. coli culture media.
- Isolated DNA can be used in PCR, cloning, sequencing, cell transfection, or enzymatic analysis without further manipulation.
Key Features
- Simple: Clearance and DNA binding in one step.
- High purity and yields: The kit utilizes a glass microfiber membrane and a clear filter column to increase the purity and yields of the plasmid.
- Rapid: The clear filter column is assembled with the spin column to combine the lysate clearance and plasmid DNA binding into one step. The 10 minutes or 50% of the time is saved when compared with similar products on the market.
Kit Contents
| Part | TBS6011-50 | TBS6011-200 |
|---|---|---|
| Mini Column with collecting tube | 50 | 200 |
| Clear Filter Column | 50 | 200 |
| Buffer 1 (B1) | 20mL | 60mL |
| Buffer 2 (B2) | 20mL | 60mL |
| Buffer 3 (B3) | 25mL | 90mL |
| Buffer 4 (B4) | 19mL | 69mL |
| Buffer 5 (B5) | 12mL | 50mL |
| Buffer 6 (B6) | 15mL | 30mL |
| RNase A (20 mg/mL) | 0.1mL | 0.3mL |
Storage Conditions
Plasmid DNA Rapidprep Mini Kit is shipped at room temperature. Buffer 1 with RNase A should be kept at 4°C. All other components are stable at room temperature.
Shelf life is 12 months after receiving.
Additional information
| SIZE | 200 Sample, 50 Samples |
|---|
Castel, P., Holtz-Morris, A., Kwon, Y., Suter, B. P., & McCormick, F. (2021). DoMY-Seq: A yeast two-hybrid-based technique for precision mapping of protein-protein interaction motifs. The Journal of biological chemistry, 296, 100023. https://doi.org/10.1074/jbc.RA120.014284
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