Exgene Viral DNA/RNA Isolation Kit

Description

The Exgene™ Viral DNA/RNA kit provides fast and easy methods for the purification of total nucleic acids from viral samples such as cell-free fluid, cell-culture supernatant, plasma, serum, swab, urine, and virus-infected samples. Purified nucleic acids can be used directly for PCR, qPCR, RT-PCR, or any downstream application without further manipulation. Exgene™ Viral DNA/RNA kit utilizes advanced silica-binding technology to purify total nucleic acids sufficiently pure for many applications. Viral samples are lysed in an optimized buffer containing detergent and lytic enzyme. Under the optimized binding conditions, nucleic acids in the lysate bind to the silica membrane and impurities pass through the membrane into a collection tube. The membranes are washed with a series of alcohol-containing buffers to remove any traces of proteins, cellular debris, and salts. Finally, pure nucleic acids are released into a clean collection tube with deionized water or low ionic strength buffer. The elute should be treated with care because nucleic acids are very sensitive to contaminants, such as nucleases, often found on general labware and dust. To ensure nucleic acid stability, it is recommended to store at 4℃ for immediate analysis or to freeze at -70℃ for long-term storage.

Kit Sizes

128-150: 50 samples

Applications

Purification of total nucleic acids (DNA/ RNA) from viral samples such as cell-free fluid, cell-culture supernatant, plasma, serum, swab, urine, and virus-infected samples.

Key Features

• Spin column format.
• Stable and consistent yield.
• Preparation time: ~ 20 minutes.
• No phenol/chloroform extraction.
• No ethanol precipitation.
• Microcolumn & carrier RNA enhance the performance of viral sample extraction.
• Various viral samples: cell-free fluid, cell-culture supernatant, plasma, serum, swab, urine, and virus-infected samples.
• Ready for use in PCR, RT-PCR, real-time PCR, and other analytical procedures.

 

Storage Conditions

All components of GeneAll® Exgene™ Viral DNA/RNA kit should be stored at room temperature (15~25℃). After reconstitution of proteinase K with storage buffer, it should be stored under 4℃ for conservation of activity. It can be stored at 4℃ for 1 year without a significant decrease in activity. But for prolonged preservation of activity, storing under -20℃ is recommended. Also, dissolved carrier RNA should be immediately used for experiments or frozen in aliquots at -20℃. Under cool ambient conditions, a precipitate can be formed in buffer BL. In such a case, heat the bottle above 37℃ to dissolve completely. GeneAll® Exgene™ Viral DNA/RNA kit is guaranteed until the expiration date printed on the product label.

Additional information

SIZE	50 Samples

5. Exgene Viral DNA RNA ver 1.1_en