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Isolation of high quality RNA is an important first step in gene expression studies such as RT-PCR, qRT-PCR, and array analysis. The Tribo™ Tissue RNA Isolation Kit is designed to isolate total RNA from tissue. Under acidic conditions RNA remains in the upper aqueous phase, while DNA and proteins remain either in the interphase or in the lower organic phase. Total RNA can then be recovered by precipitation with isopropanol and can be used for several applications. This kit can significantly improve the quantity and quality of RNA.
Direct Assays: total RNA isolation from tissue sample.
The amount of RNA isolated depends on the tissue used for isolation. The yield of total RNA from 100mg of muscle tissue should range from 100 to 150 µg and up to 800 µg can be isolated from 100 mg of liver tissue. The A260/A280 ratio of the isolated RNA should be above 1.8.
Simple and Convenient: There is no special equipment need.
High yield rate: This method gets a higher yield rate of RNA than spin column method.
Versatility: multiple RNAs include microRNA.
This kit is shipped at room temperature. Store the reagent at 4℃.
Shelf life: 12 months after receiving