CRISPR Genomic Cleavage Detection Kit (TBS6096)
$216.00
| SKU | Stock | SIZE | Price | Quantity | ||
|---|---|---|---|---|---|---|
| TBS6096 | Yes | 100 Reactions | $216.00 |
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Description
The CRISPR Genomic Cleavage Detection Kit is designed as a simple, rapid assay to verify your genomic editing process. CRISPR-edited cell samples are used as a template in PCR reactions targeting the specific region of interest. The products are then denatured and re-annealed to produce mismatches in the double strand. These mismatches are recognized and subsequently cleaved by the detection enzyme to produce product sizes that are easily distinguishable upon gel analysis. Included in the kit is a control template with the size of 750 bp after PCR amplification and of 500 bp and 250 bp after the cleavage assay.
Component
| Product Component | Quantity |
| Cell Lysis Buffer | 1.25 ml |
| Protein Degrader | 100 μl |
| Detection Enzyme | 50 μl |
| 10X Detection Buffer | 200 μl |
| Control Primer & Template | 10 μl |
| Pro Fidelity 2X PCR MasterMix | 2 x 1.25 ml |
Additional Materials Required
- Target-Specific Primers
- CRISPR-Edited Cells
- Wild-Type Cells
- Nuclease-Free H₂O
Store at -20°C.
Research use only.
Additional information
| SIZE | 100 Reactions |
|---|
Datasheet:
TBS6096-CRISPR Genomic Cleavage Detection Kit_datasheet
MSDS:
TBS6096_CRISPR Genomic Cleavage Detection Kit_MSDS-2024
Research use only.
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