CRISPR Cas9 Cleavage Detection Kit (TBS6097)

Description

CRISPR Cas9 Cleavage Detection Kit is a robust and precise RNA-dependent assay used to identify successful insertions and/or deletions in genomic DNA following CRISPR/Cas9 experiments. CRISPR/Cas9 can introduce insertion or deletion mutations (indels) on either one or both copies of a given mammalian gene. The Kit can easily identify if a given clone is monoallelic (has mutations in one copy), biallelic (both copies mutated) or is unedited (Wild-Type) which is considered a major screening advantage over the standard T7E1 Surveyor Assay. This kit uses PCR to first amplify the sgRNA target site from CRISPR edited cells; this amplicon is subsequently cleaved by a ribonucleoprotein (RNP) complex which generates a unique pattern that can be easily resolved on agarose gel and interpreted by the user. Using this kit after gene editing saves time and energy from the arduous screening process and provides clear genotyping results.

Component

Additional Materials Required

• Target-Specific Primers
• CRISPR-Edited Cells
• Nuclease-Free H₂O
• sgRNA Target-Specific Oligo
• Wild-Type Cells

Store at -20°C.

Research use only.

Additional information

Datasheet:

TBS6097-CRISPR Cas9 Cleavage Detection Kit_datasheet

MSDS:

TBS6097_CRISPR Cas9 Cleavage Detection Kit_MSDS

Research use only.