Ultra-Sensitive Human IFN gamma ELISA Kit (TBS3230U)

Description

The Tribio® Fast Human IFN- γ ELISA Maxi is a solid phase ELISA designed to measure human IFN- γ levels in cell culture supernatants, serum, and plasma. The main feature is that the kit uses our novel proprietary approaches to combine samples and detection into a one-step instead of the complicated traditional methods. It makes the assay simple, easy, accurate and fast. The measurement can be finished in 3 hours, no need for 5-6 hours (Fig. 1). The detection range is from 6 to 4860 pg/mL. The levels of human IFN- γ samples are parallel to the standard curves obtained using the kit standards linearly. These results indicate that this kit can be used to determine relative mass values for natural human IFN- γ protein.

 

Typical Data

This standard curve is provided for demonstration only as Fig.3. A standard curve should be generated for each set of samples assayed.

Sensitivity

The minimum detectable dose (MOD) of human IFN-γ is typically 6 pg/ml.

Specificity

This assay recognizes natural and recombinant human IFN- γ.

 

Simple Procedures (Fig. 1)

 

Simple Standard Preparation

 

Accurate Standard Curve

 

Kit Content and Storage Conditions

Part	Part#	Description	Storage of Opened/Reconstituted
HumanIFN- γ Microplate	TBS3230UA	96 well strip microplate (12 strips of 8 wells) coated with a polyclonal antibody specific for human IFN- γ.	Return unused wells to the foil pouch. Reseal along the entire edge of the zip-seal. May be stored for up to 1 month at 2-8 °C.
HumanIFN- γ Standard	TBS3230UB	50 µL of Recombinant human IFN- γ protein (145.8ng/mL).	Aliquot and store at -20 ℃ for up to 1 month in a manual defrost freezer. Avoid repeated freeze-thaw cycles.
Detection A	TBS3230UC	2.2 mL of HRP-Human IFN- γ antibody.	May be stored for up to 3 months at 2-8 °C.
Assay Diluent	TBS3230UD	12 mL of a buffered protein base with preservatives.
Wash Buffer	TBS3000W	12 mL of concentrated solution (10x).
TMB Substrate	TBS3000T	12 mL of ultra-sensitive TMB substrate.
Stop Solution	TBS3000S	6 mL of 2 N sulfuric acid.

Store the unopened kit at 2-8℃. Do not use past kit expiration date.
The kit contains sufficient materials to run an ELISA on one 96 well plate.

Additional information

SIZE	1 x 96-well plate

Datasheet:

TBS3230U _human_IFN_gamma_Ultra-Sensitive elisa_V01_2024

MSDS:

TBS3230U_HumanIFN- γ_ELISA_MSDS

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