Botulinum Neurotoxin Type A (BoNT-A) ELISA (TBS31007)

Description

Tribioscience’s Botulinum Neurotoxin Type A (BoNT-A) ELISA is designed to quantitatively detect Botulinum Neurotoxin Type A (BoNT-A) levels in serum, plasma, and other biological samples. The main feature is that the kit uses our novel proprietary approaches to combine samples and detections into a one-step instead of the complicated traditional methods. It makes the assay simple, easy, accurate, and fast (Fig. 1). The detection range is from 46.9 to 3000 pg/mL. The levels of Botulinum Neurotoxin Type A (BoNT-A) samples are parallel to the standard curves obtained using the kit standards linearly. Therefore, the kit can be used to determine relative mass values for natural Botulinum Neurotoxin Type A (BoNT-A) protein.

 

Typical Data

This standard curve (R²=0.9972) is provided for demonstration only. A standard curve should be generated for each set of samples assayed. Fig. 3 is an example of typical Data.

Sensitivity

The minimum detectable dose (MOD) of BoNT-A is typically 46 pg/mL.

The Intra-assay CV and the Inter-assay CV are ˂10%.

Specificity

This assay recognizes natural and recombinant BoNT-A.

No cross-reactivity with others.

 

Simple Procedures (Fig. 1)

 

Simple Standard Preparation 

 

Accurate Standard Curve

 

Kit Content and Storage Conditions

Part	Part#	Description	Storage of Opened/Reconstituted
Botulinum Neurotoxin Type A Microplate	TBS31007A	96-well polystyrene microplate (12 strips of 8 wells) coated with a monoclonal antibody specific for BoNT-A.	Return unused wells to the foil pouch. Reseal along the entire edge of the zip-seal. May be stored for up to 1 month at 2-8 °C.
Botulinum Neurotoxin Type A Standard	TBS31007B	50 µl of Recombinant Botulinum Neurotoxin Type A (30 ng/mL).	Aliquot and store at -20 ℃ for up to 1 month in a manual defrost freezer. Avoid repeated freeze-thaw cycles.
Detection A	TBS31007C	2.1 mL of biotin-Botulinum Neurotoxin Type A antibody.	May be stored for up to 3 months at 2-8 °C.
Detection B	TBS31007D	200 µL of streptavidin HRP.
Assay Diluent	TBS31007E	25 mL of a buffered protein base with preservatives.
10x Wash Buffer	TBS3000W	12 mL of concentrated solution (10x).
TMB Substrate	TBS3000T	12 mL of ultra-sensitive TMB substrate.
Stop Solution	TBS3000S	6 mL of 2 N sulfuric acid.

Store the unopened kit at 2-8 ℃. Do not use past kit expiration dates.
The kit contains sufficient materials to run an ELISA on one 96-well plate.

 

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Additional information

SIZE	1 x 96-well plate

Datasheet:

TBS31007 Botulinum Neurotoxin Type A (BoNT-A) Elisa datasheet 6-2023 (3)

MSDS:

TBS31007_Botulinum Neurotoxin_ELISA_MSDS