Tribo™ Mouse TNF-α ELISA Kit

Tribo™ Mouse TNF-α ELISA Kit

$320.00$560.00

Tumor necrosis factor alpha (TNF-α) is a potent mediator of immune and inflammatory response. It is produced by many activated cell types including monocytes, macrophages, astrocytes, granulocytes, T and B lymphocytes, NK cells, keratinocytes, fibroblasts, and certain tumor cells.

SKU: TBS3050 Category:
SKUStock SIZE PriceQuantity
TBS3050-01Yes1x96-well plate $320.00
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TBS3050-02Yes2x96-well plate $560.00
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Description

Tribo™ Mouse TNF-α ELISA Kit

Tumor necrosis factor-alpha (TNF-α) is a potent mediator of immune and inflammatory response. It is produced by many activated cell types including monocytes, macrophages, astrocytes, granulocytes, T and B lymphocytes, NK cells, keratinocytes, fibroblasts, and certain tumor cells. TNF-α is involved in a number of pathological conditions including inflammation, apoptosis, lipid metabolism, trauma, asthma, rheumatoid arthritis, pain, obesity septic shock, autoimmunity, and cancer.

The TriboTM Fast Mouse TNF-α ELISA is designed to quantitatively detect mouse TNF-α levels in different tissues including skin, muscle, neural, serum, and other biological samples. The main feature is that the kit uses our novel proprietary approaches to combine samples and detections into a one-step instead of the complicated traditional methods. It makes the assay simple, easy, accurate, and fast. The measurement can be finished in 1 hour, not need 4-5 hours (Fig. 1). The detection arrange is from 15 to 1000pg/mL. The levels of mouse TNF-α samples are parallel to the standard curves obtained using the kit standards linearly. These results indicate that this kit can be used to determine relative mass values for natural mouse TNF-α protein.

Kit Size:

1x 96-well plate (8×12 strip well).
2x 96-well plate (8×12 strip well).

Applications:

Direct Assays: quantitative TNF-α in plasma and serum.

Key Features:

  • Sensitivity: The minimum detectable dose of mouse TNF-α is less than 8.0 pg/mL typically.
  • Specificity: This assay recognizes recombinant and natural mouse TNF-α. No cross-reactivity with any of other cytokine is observed.

 

Kit Contents:

ReagentsQuantity
Mouse TNF-α Microplate96 well polystyrene microplate (12 strips of 8 wells) coated
with a polyclonal antibody specific for mouse TNF-α.
Mouse TNF-α Standard20ul of Recombinant mouse TNF-α protein (100ug/mL).
Detection A2.2 ml of Biotin-mouse TNF-α antibody.
Detection B12 ml of Streptavidin-HRP.
Assay Diluent12 ml of a buffered protein base with preservatives.
WashBuffer12ml of concentrated solution(10x).
TMB Substrate12 ml of ultra-sensitive TMB substrate.
Stop Solution6ml of 2 N sulfuric acid.

 

Storage Conditions:

Store the kit at 4℃ and standard at -20℃, respectively.
Shelf life: 12 months after receiving

 

Fig. 1 Simple Assays Procedures

 

Fig.2 Diagram for Mouse TNF-α standard preparation

Fig.3 Fast Mouse TNF-α ELISA

Related Products:

  1. Tribo™ Mouse IL-1β ELISA Kit (TBS3030)
  2. Tribo™ Mouse IL-2 ELISA Kit (TBS3031)
  3. Tribo™ Mouse IL-4 ELISA Kit (TBS3032)
  4. Tribo™ Mouse IL-6 ELISA Kit (TBS3040)
  5. Tribo™ Mouse IL-10 ELISA Kit (TBS3044)

 

Additional information

SIZE

1×96-well plate, 2×96-well plate

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