Tribo™ Mouse TNF-α ELISA Kit

$320.00$560.00

Tumor necrosis factor alpha (TNF-α) is a potent mediator of immune and inflammatory response. It is produced by many activated cell types including monocytes, macrophages, astrocytes, granulocytes, T and B lymphocytes, NK cells, keratinocytes, fibroblasts, and certain tumor cells.

SKU: TBS3050 Category:
SKUStock SIZE PriceQuantity
TBS3050-01 Yes 1 x 96-well plate $320.00
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TBS3050-02 Yes 2 x 96-well plate $560.00
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Description

Tribioscience’s Fast Mouse TNF-α ELISA is designed to quantitatively detect mouse TNF-α levels in different tissues including skin, muscle, neural, serum, and other biological samples. The main feature is that the kit uses our novel proprietary approaches to combine samples and detections into a one-step instead of the complicated traditional methods. It makes the assay simple, easy, accurate, and fast. The measurement can be finished in 2 hours, with no need for 4-5 hours (Fig. 1). The detection range is from 31 to 2000 pg/mL. The levels of mouse TNF-α samples are parallel to the standard curves obtained using the kit standards linearly. These results indicate that this kit can be used to determine relative mass values for natural mouse TNF-α protein.

 

Typical Data

This standard curve (R2=0.9998) is provided for demonstration only. A standard curve should be generated for each set of samples assayed. Fig. 3 is an example of typical Data.

Sensitivity

The minimum detectable dose (MOD) of mouse TNF-α is typically 10 pg/ml.

The Intra-assay CV is 3.79% the Inter-assay CV is ˂10%.

Specificity

This assay recognizes natural and recombinant mouse TNF-α.
No cross-reactivity: human TNF- α, β; human TNF RI, human TNF RII; Porcine TNF-α; Rat TNF-α; Mouse TNF RI, Mouse TNF RII.

 

Simple Procedures (Fig. 1)

Simple Standard Preparation (Fig.2)

 

Accurate Standard Curve (Fig.3 )

Kit Content and Storage Conditions

Part Part# Description Storage of Opened/Reconstituted
Mouse TNF-α Microplate TBS3050A 96 well polystyrene microplate (12 strips of 8 wells) coated with a polyclonal antibody specific for mouse TNF-α. Return unused wells to the foil pouch. Reseal along the entire edge of the zip-seal. May be stored for up to 1 month at 2-8 °C.
Mouse TNF-α Standard TBS3050B 20 µL of Recombinant mouse TNF-α protein (100 ng/mL). Aliquot and store at -20 ℃ for up to 1 month in a manual defrost freezer. Avoid repeated freeze-thaw cycles.
Detection A TBS3050C 2.2 mL of Biotin-mouse TNF-α antibody. May be stored for up to 3 months at 2-8 °C.
Detection B TBS3050D 200 µL of Streptavidin-HRP.
Assay Diluent TBS3050E 25 mL of a buffered protein base with preservatives.
Wash Buffer TBS3000W 12 mL of concentrated solution (10x).
TMB Substrate TBS3000T 12 mL of ultra-sensitive TMB substrate.
Stop Solution TBS3000S 6 mL of 2 N sulfuric acid.

Store the unopened kit at 2-8 ℃. Do not use past kit expiration dates.
The kit contains sufficient materials to run an ELISA on one 96-well plate.

 

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  3. Tribo™ Mouse IL-4 ELISA Kit (TBS3032)
  4. Tribo™ Mouse IL-6 ELISA Kit (TBS3040)
  5. Tribo™ Mouse IL-10 ELISA Kit (TBS3044)

Additional information

SIZE

1 x 96-well plate, 2 x 96-well plate

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