Tribo™ Mouse TNF-α ELISA Kit
$320.00 – $560.00
Tumor necrosis factor alpha (TNF-α) is a potent mediator of immune and inflammatory response. It is produced by many activated cell types including monocytes, macrophages, astrocytes, granulocytes, T and B lymphocytes, NK cells, keratinocytes, fibroblasts, and certain tumor cells.
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Tribo™ Mouse TNF-α ELISA Kit
Tumor necrosis factor-alpha (TNF-α) is a potent mediator of immune and inflammatory response. It is produced by many activated cell types including monocytes, macrophages, astrocytes, granulocytes, T and B lymphocytes, NK cells, keratinocytes, fibroblasts, and certain tumor cells. TNF-α is involved in a number of pathological conditions including inflammation, apoptosis, lipid metabolism, trauma, asthma, rheumatoid arthritis, pain, obesity septic shock, autoimmunity, and cancer.
The TriboTM Fast Mouse TNF-α ELISA is designed to quantitatively detect mouse TNF-α levels in different tissues including skin, muscle, neural, serum, and other biological samples. The main feature is that the kit uses our novel proprietary approaches to combine samples and detections into a one-step instead of the complicated traditional methods. It makes the assay simple, easy, accurate, and fast. The measurement can be finished in 1 hour, not need 4-5 hours (Fig. 1). The detection arrange is from 15 to 1000pg/mL. The levels of mouse TNF-α samples are parallel to the standard curves obtained using the kit standards linearly. These results indicate that this kit can be used to determine relative mass values for natural mouse TNF-α protein.
1x 96-well plate (8×12 strip well).
2x 96-well plate (8×12 strip well).
Direct Assays: quantitative TNF-α in plasma and serum.
- Sensitivity: The minimum detectable dose of mouse TNF-α is less than 8.0 pg/mL typically.
- Specificity: This assay recognizes recombinant and natural mouse TNF-α. No cross-reactivity with any of other cytokine is observed.
|Mouse TNF-α Microplate||96 well polystyrene microplate (12 strips of 8 wells) coated|
with a polyclonal antibody specific for mouse TNF-α.
|Mouse TNF-α Standard||20ul of Recombinant mouse TNF-α protein (100ug/mL).|
|Detection A||2.2 ml of Biotin-mouse TNF-α antibody.|
|Detection B||12 ml of Streptavidin-HRP.|
|Assay Diluent||12 ml of a buffered protein base with preservatives.|
|WashBuffer||12ml of concentrated solution(10x).|
|TMB Substrate||12 ml of ultra-sensitive TMB substrate.|
|Stop Solution||6ml of 2 N sulfuric acid.|
Store the kit at 4℃ and standard at -20℃, respectively.
Shelf life: 12 months after receiving
Fig. 1 Simple Assays Procedures
Fig.2 Diagram for Mouse TNF-α standard preparation
Fig.3 Fast Mouse TNF-α ELISA
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1×96-well plate, 2×96-well plate