Tribo™ Mouse IL-10 ELISA Kit (TBS3044)

Description

Tribioscience’s Mouse IL-10 Fast ELISA kit is designed to quantitatively detect mouse IL-10 levels in tissues, serum, plasma, and other biological samples. The main feature is that the kit uses our novel proprietary approaches to combine samples and detections into a one-step instead of the complicated traditional methods. It makes the assay simple, easy, accurate, and fast (Fig. 1). The detection range is from 31 to 2000 pg/mL. The levels of mouse IL-10 samples are parallel to the standard curves obtained using the kit standards linearly. Therefore, the kit can be used to determine relative mass values for natural mouse IL-10 protein.

 

Typical Data

This standard curve (R²=0.9997) is provided for demonstration only. A standard curve should be generated for each set of samples assayed. Fig. 3 is an example of typical Data.

Sensitivity

The minimum detectable dose (MOD) of mouse IL-10 is typically 31 pg/ml.
The Intra-assay CV and the Inter-assay CV are ˂10%.

Specificity

This assay recognizes natural and recombinant mouse IL-10.
No cross-reactivity with human, canine, feline and porcine IL-10.

 

Simple Procedures (Fig. 1)

 

Simple Standard Preparation

 

Accurate Standard Curve

 

Kit Content and Storage Conditions

Part	Part#	Description	Storage of Opened/Reconstituted
Mouse IL-10 Microplate	TBS3044A	96 well polystyrene microplate (12 strips of 8 wells) coated with a monoclonal antibody specific for mouse IL-10.	Return unused wells to the foil pouch. Reseal along the entire edge of the zip-seal. May be stored for up to 1 month at 2-8 °C.
Mouse IL-10 Standard	TBS3044B	20 µL of Recombinant mouse IL-10 (200 ng/mL).	Aliquot and store at -20 ℃ for up to 1 month in a manual defrost freezer. Avoid repeated freeze-thaw cycles.
Detection A	TBS3044C	2.1 mL of biotin-mouse IL-10 antibody.	May be stored for up to 3 months at 2-8 °C.
Detection B	TBS3044D	200 µL of streptavidin HRP (80x).
Assay Diluent	TBS3044E	25 mL of a buffered protein base with preservatives.
Wash Buffer	TBS3000W	12 mL of concentrated solution (10x).
TMB Substrate	TBS3000T	12 mL of ultra-sensitive TMB substrate.
Stop Solution	TBS3000S	6 mL of 2 N sulfuric acid.

Store the unopened kit at 2-8 ℃. Do not use past kit expiration dates.
The kit contains sufficient materials to run an ELISA on one 96-well plate.

 

Additional information

SIZE	1 x 96-well plate

Datasheet:

TBS3044 Mouse IL-10 Elisa datasheet_V02

MSDS:

TBS3044_Mouse IL-10_ELISA_MSDS