Tribo™ Mouse IFN γ ELISA Kit

Tribo™ Mouse IFN γ ELISA Kit

$320.00

Interferon-gamma (IFN-γ, also known as type II interferon) is an important immunoregulatory cytokine through its antiviral activity. IFN- γ is produced by a few cell types under inflammatory conditions, including dendritic epidermal cells, T cells, keratinocytes, peripheral blood T cells, mast cells, neurons, CD8+T cells, macrophages, B cells, neutrophils, NK cells, CD4+T cells, and testicular spermatids. It plays key roles in host defense, and the progression of inflammatory diseases such as autoimmunity and atherosclerosis by exerting anti-viral, anti-proliferative, and immunoregulatory activities.

SKU: TBS3084 Category:
SKUStock SIZE PriceQuantity
TBS3084Yes1x96-well plate $320.00
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Description

Tribo™ Mouse IFN γ ELISA Kit

Interferon-gamma (IFN-γ, also known as type II interferon) is an important immunoregulatory cytokine through its antiviral activity. IFN- γ is produced by a few cell types under inflammatory conditions, including dendritic epidermal cells, T cells, keratinocytes, peripheral blood T cells, mast cells, neurons, CD8+T cells, macrophages, B cells, neutrophils, NK cells, CD4+T cells, and testicular spermatids. It plays key roles in host defense, and the progression of inflammatory diseases such as autoimmunity and atherosclerosis by exerting anti-viral, anti-proliferative, and immunoregulatory activities.

The Fast Mouse IFN-γ ELISA is a solid phase ELISA designed to measure mouse IFN-γ levels in cell culture supernatants, serum, and plasma. The main feature is that the kit uses our novel proprietary approaches to combine samples and detections into a one-step instead of the complicated multiple steps in traditional methods. It makes the assay simple, easy, accurate, and fast. The measurement can be finished in 2 hours, not need 4-5 hours (Fig. 1). The detection arrangement is from 8 to 2000pg/mL. The levels of mouse IFN-γ samples are parallel to the standard curves obtained using the kit standards linearly. These results indicate that this kit can be used to determine relative mass values for natural mouse IFN-γ protein.

Kit Contents:

ReagentsDescription
Mouse IFN- γ Microplate96 well microplate (12 strips of 8 wells) coated with a
Capture Antibody specific for mouse IFN- γ.
Mouse IFN- γ Standard30 µl of Recombinant mouse IFN- γ protein (100ng/mL).
Detection A2.1 ml of mouse IFN- γ antibody.
Detection B12 ml of Streptavidin-HRP
Assay Diluent15 ml of a buffered protein base with preservatives.
10x WashBuffer12ml of concentrated solution(10x).
TMB Substrate 12 ml of ultra-sensitive TMB substrate.
Stop Solution 6ml of 2 N sulfuric acid.

Store the unopened kit at 2-8 ℃. Do not use past kit expiration date.
The kit contains sufficient materials to run an ELISA on one 96 well plate.

 

Fig.1 Simple Assay Procedures

 

Fig. 2 Diagram for Mouse IFN-γ standard preparation

Fig.3 Mouse IFN-γ standard curve

Related Products:

  1. Tribo™ Mouse IL-1β ELISA Kit (TBS3030)
  2. Tribo™ Mouse IL-2 ELISA Kit (TBS3031)
  3. Tribo™ Mouse IL-4 ELISA Kit (TBS3032)
  4. Tribo™ Mouse IL-6 ELISA Kit (TBS3040)
  5. Tribo™ Mouse IL-10 ELISA Kit (TBS3044)

 

Additional information

SIZE

1×96-well plate

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