RIPA Buffer

RIPA Buffer

$39.00

RadioImmunoPrecipitation Assay (RIPA) Buffer is used to lyse cells and tissues under nondenaturing conditions.

SKU: TBS5017 Category:
SKUStock SIZE (ml) PriceQuantity
TBS5017Yes50 mL/each $39.00
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Description

RadioImmunoPrecipitation Assay (RIPA) Buffer is used to lyse cells and tissues under nondenaturing conditions.

Compoents in 1x RIPA Buffer:

  • 20mM Tris-HCl (pH 7.5)
  • 150mM NaCl
  • 1mM Na2EDTA
  • 1mM EGTA 1% NP-40
  • 2.5mM sodium pyrophosphate
  • 1mM β-glycerophosphate
  • 1mM Na3VO4 1 µg/ml leupeptin

 

Directions for Use:

  • Aliquotting of RIPA buffer is recommended if many small experiments are to be performed. For longer periods of time, buffer should be stored at –20°C. If buffer will be continually used, it is recommended that the buffer be kept at 4°C for 1-2 weeks.
  • Thaw RIPA buffer at room temperature, mixing end-over-end.
  • Chill RIPA buffer on ice and add PMSF (final 1 mM) just prior to use.

 

Storage Conditions:

Supplied as a ready to use solution, 50mL. 100mM PMSF, 0.5mL.
Store at –20℃. For short-term storage (1-2 weeks), RIPA Buffer can be stored at 4͓.

Additional information

SIZE (ml)

50 mL/each

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