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DNA Loading Buffer is a complete solution for the preparation of DNA samples prior to agarose gel electrophoresis and polyacrylamide gel electrophoresis. This optimized loading buffer prevents DNA degradation during the electrophoresis run.
Components in 10x loading buffer:
- 0.1M Tris-HCl.
- 0.6% SDS
- 0.1% Bromophenol blue
- 0.1% Xylene cyanol
- 30% Glycerol
Directions for Use:
Mix 1-volume DNA loading buffer with 9-volume DNA sample, loading to agarose or polyacrylamide gel.
Supplied as a 10X solution, 5x 1mL.
Store at 4°C or room temperature.