Glutathione (GSH) Fluorometric Assay (TBS2028)

Description

Thiol groups, found as free cysteine, glutathione (GSH), and cysteine residues in proteins, are involved in many biological processes. The detection and measurement of free thiols is one essential task for investigating biological processes. Thiols are extremely efficient antioxidants which can protect cellular lipids, proteins and nucleic acids against peroxidative damage due to their strong reductive capacity and their ability to react with free radicals.

Tribioscience’s GSH Quantification Fluorometric Assay Kit provides a flexible, accurate, sensitive, and time-saving approach for detecting GSH in a wide variety of samples.

In this assay, the non-fluorescent probe binds to the thiol in the sample to produce a fluorescent product which can be measured at 400 nm excitation, 460 nm emission.

Features

• Flexible: Suitable for 96-well and 384-well plate.
• Accuracy: Fluorescent measurement is proportional to the free thiol content.
• Sensitive: detection range from 0.3 µM to 20 µM.
• Time-saving: Just incubate 10 minutes and read out style.

Applications

• Measure free thiol group in a variety samples like cells and tissues.

Typical GSH Standard Curve

Research use only.

Additional information

Datasheet:

TBS2028 GSH Fluorescent Quantification Assay_V01_8-2023

MSDS:

TBS2028-GSH Fluorometric Assay_MSDS_2024

Research use only.