Glutamate Dehydrogenase (GDH) Colorimetric Assay (TBS2025)

Description

Glutamate dehydrogenase (GDH) is a mitochondria enzyme and related to amino acids metabolism. GDH is involved in the metabolism of glutamate, which is essential for neurotransmitter recycling and overall brain function. Elevated levels of GDH in the blood can indicate liver damage, and its activity is often used in clinical diagnostics. Dysfunction of GDH is linked to conditions such as Parkinson’s disease, schizophrenia, and frontotemporal dementia.

Tribio™ Glutamate dehydrogenase (GDH) activity Quantification Colorimetric Assay is based on a dehydrogenase coupled reaction convert chromophore to a colored formazan, which can be measured at OD 460 nm. The generated signal is proportional to the content of NADPH. This assay specifically detects GDH activity from the cell lysate. The kit provides the easiest and most accurate approach to measure GDH activity from a variety of samples.

Applications

Specifically detect glutamate dehydrogenase activity from cell and biological tissues.

Kit Contents for 100 tests:

Storage conditions: Store the Reagent at –20°.

Typical standard curve showed as an example in Fig.1:

Typical positive enzyme control assay curve showed as an example in Fig.2:

Research use only.

Additional information

Datasheet:

TBS2025_Glutamate dehydrogenase activity_Colorimetric Assay_data_sheet_V03-Final

MSDS:

TBS2025-GDH Colorimetric Assay_MSDS_2024

Research use only.