647 Red EdU Click Cell Proliferation Assay (TBS2044)

$460.00$736.00

Synonyms: 5-EdU, EdU, 2′-Deoxy-5-ethynyluridine, red fluorescence

SKU: TBS2044 Category:
SKUStock SIZE (test) PriceQuantity
TBS2044-50 Yes 50 Tests $460.00
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TBS2044-100 Yes 100 Tests $736.00
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Description

5-ethynyl-2´-deoxyuridine (EdU) is a thymidine analog. It gets incorporated into the newly synthesized DNA of proliferating cells in the place of thymidine. EdU incorporated into the cells can be used directly as an indicator of cell proliferation.

 

Tribioscience’s EdU Click Cell Proliferation Assay Kit detects EdU incorporated with fluorescent labeling via a copper-catalyzed click reaction with a 647-azide red fluorescence. Compared to other cell proliferation assays, this kit detects only the proliferating cells and not the seeded cells. This kit can detect as few as 50-100 proliferating cells and enables rapid, sensitive, and multiplex-compatible detection of proliferating cells via DNA synthesis.

 

EdU Click Cell Proliferation Assay Kit provides a precise, fast and simple method to quantitate cell proliferation based on the measurement of EdU incorporation during DNA synthesis (S phase) in proliferating cells.

 

Kit Components and Storage for 50 or 100 tests

Part Name 50 tests 100 tests
EdU stock solution (500 x) 12µL 24 µL
647 Red Fluorescence Stock Solution (500x) 11 µL 22 µL
Enhancer (10x) 25 mg 50 mg
FixDenat (1x) 13 mL 26 mL
Wash Reagent (10x) 3 mL 6 mL
Catalyst Solution (25x) 205 µL 410 µL
Click Reaction Buffer 5 mL 10 mL

Storage: -20 °C

 

Applications

  • Detection and quantification of cell proliferation induced by growth factors, cytokines, mitogens, and nutrients.
  • Analysis of cytotoxic and cytostatic compounds such as anticancer drugs, toxic agents and other pharmaceuticals.
  • Determination of the inhibitory or stimulatory effects of various compounds on cell proliferation.

 

Sample Types

  • Adherent and Suspension cells.

Research use only.

Additional information

SIZE (test)

100 Tests, 50 Tests

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